Tracing the metabolism of HT-2 toxin and T-2 toxin in barley by isotope-assisted untargeted screening and quantitative LC-HRMS analysis

Analytical and Bioanalytical Chemistry
Jacqueline Meng-ReitererRainer Schuhmacher

Abstract

An extensive study of the metabolism of the type A trichothecene mycotoxins HT-2 toxin and T-2 toxin in barley using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) is reported. A recently developed untargeted approach based on stable isotopic labelling, LC-Orbitrap-MS analysis with fast polarity switching and data processing by MetExtract software was combined with targeted LC-Q-TOF-MS(/MS) analysis for metabolite structure elucidation and quantification. In total, 9 HT-2 toxin and 13 T-2 toxin metabolites plus tentative isomers were detected, which were successfully annotated by calculation of elemental formulas and further LC-HRMS/MS measurements as well as partly identified with authentic standards. As a result, glucosylated forms of the toxins, malonylglucosides, and acetyl and feruloyl conjugates were elucidated. Additionally, time courses of metabolite formation and mass balances were established. For absolute quantification of those compounds for which standards were available, the method was validated by determining apparent recovery, signal suppression, or enhancement and extraction recovery. Most importantly, T-2 toxin was rapidly metabolised to HT-2 toxin and for both parent toxins HT-2 ...Continue Reading

References

May 1, 1990·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M GareisB Gedek
Sep 1, 1988·Applied and Environmental Microbiology·C J MirochaG Bean
Feb 19, 2003·Phytochemistry·Lloyd W SumnerRichard A Dixon
Nov 6, 2004·Nature Biotechnology·Patrick G A PedrioliRuedi Aebersold
Apr 12, 2007·Journal of Experimental Botany·Daisuke MasudaTakumi Nishiuchi
Jul 9, 2008·Bioinformatics·Darren KessnerParag Mallick
Sep 1, 2011·Toxicology Letters·Franz BerthillerGerhard Adam
Nov 22, 2011·The Analyst·Aihua ZhangXijun Wang
Sep 27, 2012·Analytical and Bioanalytical Chemistry·C BueschlR Schuhmacher
Oct 11, 2012·Molecular Nutrition & Food Research·Franz BerthillerJoerg Stroka
Nov 20, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Elisabetta De AngelisAngelo Visconti
Dec 19, 2014·Journal of Agricultural and Food Chemistry·Susan P McCormickCletus P Kurtzman
Aug 19, 2015·Journal of Agricultural and Food Chemistry·Alexis V NathanailFranz Berthiller

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Citations

Aug 19, 2015·Journal of Agricultural and Food Chemistry·Alexis V NathanailFranz Berthiller
Sep 10, 2016·Current Opinion in Biotechnology·Dana M Freund, Adrian D Hegeman
Apr 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcin BryłaRenata Jędrzejczak
Feb 8, 2018·Analytical and Bioanalytical Chemistry·Li LiLiru Xia
Oct 6, 2019·Scientific Reports·Laura RighettiChiara Dall'Asta
Dec 9, 2016·Toxins·Jacqueline Meng-ReitererRainer Schuhmacher
May 1, 2018·Analytical and Bioanalytical Chemistry·Laura RighettiChiara Dall'Asta
Jan 26, 2017·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Jan Alexander
Oct 23, 2019·Analytical and Bioanalytical Chemistry·David StadlerRudolf Krska
Nov 4, 2016·Journal of the Science of Food and Agriculture·Li LiXizhi Jiang
Oct 2, 2020·Molecular Medicine Reports·Yuan DongHuiyan Wang
Jan 30, 2021·Frontiers in Plant Science·Laura RighettiChiara Dall'Asta
Mar 3, 2021·Food Research International·Ajibola Bamikole OyedejiOluwafemi Ayodeji Adebo
Jan 26, 2018·Journal of Agricultural and Food Chemistry·Enrico RolliRenato Bruni
Aug 9, 2017·Analytical Chemistry·Christoph BueschlRainer Schuhmacher

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Methods Mentioned

BETA
nuclear magnetic resonance
glucosylation
acetylation

Software Mentioned

Orbitrap
Thermo Xcalibur
MassHunter Quantitative Analysis
ProteoWizard
MassHunter Acquisition
MassHunter Qualitative Quantitative
MetExtract

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