Track-etched membrane microplate and smartphone immunosensing for SARS-CoV-2 neutralizing antibody.

Biosensors & Bioelectronics
Cong WangChengyao Li


The level of neutralizing antibody (NAb) to SARS-CoV-2 could be used to evaluate the acquired protective immunity of COVID-19 patients or vaccinees. Here we develop a track-etched microporous membrane filtration microplate (TEM) and optical fibers transmitted immunosensing smartphone platform (TEMFIS) based surrogate virus neutralization test (TEMFIS-sVNT) for rapid one-step testing of NAb to SARS-CoV-2. Coefficient variation (CV) of intra-assay and inter-assay precisions of TEMFIS-sVNT varied below 9% or 14%, respectively. By agreement with pseudovirus neutralization test (pVNT) and ELISA-sVNT for testing of serum samples from 41 COVID-19 patients, 50 COVID-19 vaccinees and 320 healthy blood donors (P = 0.895), TEMFIS-sVNT detected the NAb positivity (sensitivity) in 92.68% COVID-19 patients and 76% vaccinees, but the NAb negativity (specificity) in 100% blood donors. In conclusion, TEMFIS-sVNT can be used for quantitatively point-of-care testing of neutralizing antibody to SARS-CoV-2 in blood samples from COVID-19 patients and vaccinees.


Jul 15, 2005·PLoS Medicine·William R RodriguezJohn T McDevitt
Sep 1, 2016·Annual Review of Virology·Fang Li
Jan 23, 2020·Journal of Medical Virology·Yu ChenDeyin Guo
Jan 30, 2020·The New England Journal of Medicine·Qun LiZijian Feng
Feb 11, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shumei TianMing Li
Mar 4, 2020·Nature Microbiology·UNKNOWN Coronaviridae Study Group of the International Committee on Taxonomy of Viruses
Mar 11, 2020·Cell·Alexandra C WallsDavid Veesler
Mar 15, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Chun LiLinzhu Ren
Jun 1, 2020·Current Protocols in Microbiology·Emelissa J MendozaMichael Drebot
Oct 13, 2020·Emerging Microbes & Infections·Benjamin MeyerChantal Reusken
May 28, 2021·Scientific Reports·Margherita PassarielloClaudia De Lorenzo

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