Tracking antiangiogenic components from Glycyrrhiza uralensis Fisch. based on zebrafish assays using high-speed countercurrent chromatography

Journal of Separation Science
Liwen HanKechun Liu

Abstract

Natural products are some of the most important sources of lead compounds for drug discovery. The advanced isolation technique of lead compounds of natural origin using therapeutically relevant bioassays is capable of enhancing work efficiency from complex multiconstituent extracts. In the present study, a bioassay-guided isolation strategy combined with bioactivity screening was used to identify novel angiogenesis inhibitors from licorice (Glycyrrhiza uralensis Fisch.) based on the zebrafish model and rapid preparative separation by high-speed countercurrent chromatography. Zebrafish embryos at 24 h postfertilization were chosen as the angiogenesis inhibition model for bioactivity screening. A solvent system (n-hexane-ethyl acetate-methanol-water) with different ratios was optimized and applied in the high-speed countercurrent chromatography separation of two fractions, Fr5 and Fr6, from the ethyl acetate extract of licorice. Blood circulation and vascular outgrowth in intersegmental vessels were found to be simultaneously inhibited by isoliquiritigenin and isolicoflavonol in a dose-dependent manner. Thus, these two compounds were identified and considered as active inhibitors against angiogenesis. These experimental results i...Continue Reading

References

Mar 23, 2002·Current Biology : CB·Holger KnautChristiane Nüsslein-Volhard
Jul 31, 2010·Investigative Ophthalmology & Visual Science·Liming CaoChi-Pui Pang
Oct 20, 2010·Drug and Chemical Toxicology·Sifeng WangXiqiang Chen
Jul 2, 2011·The British Journal of Ophthalmology·Vishal JhanjiGary Hin-Fai Yam
Sep 8, 2011·Journal of Separation Science·Lei FangLuqi Huang

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Citations

Jul 16, 2015·Journal of Natural Products·J Brent FriesenGuido F Pauli
Dec 23, 2017·International Journal of Molecular Sciences·Ladislav MirossayJán Mojžiš
Jan 15, 2020·The American Journal of Chinese Medicine·Chengcheng WangXunhong Liu
Oct 16, 2015·Phytotherapy Research : PTR·Hossein Hosseinzadeh, Marjan Nassiri-Asl

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