Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR

Environment International
Xin-Li AnYong-Guan Zhu

Abstract

Wastewater treatment plants (WWTPs) contain diverse antibiotic resistance genes (ARGs), and thus are considered as a major pathway for the dissemination of these genes into the environments. However, comprehensive evaluations of ARGs dynamic during wastewater treatment process lack extensive investigations on a broad spectrum of ARGs. Here, we investigated the dynamics of ARGs and bacterial community structures in 114 samples from eleven Chinese WWTPs using high-throughput quantitative PCR and 16S rRNA-based Illumina sequencing analysis. Significant shift of ARGs profiles was observed and wastewater treatment process could significantly reduce the abundance and diversity of ARGs, with the removal of ARGs concentration by 1-2 orders of magnitude. Whereas, a considerable number of ARGs were detected and enriched in effluents compared with influents. In particular, seven ARGs mainly conferring resistance to beta-lactams and aminoglycosides and three mobile genetic elements persisted in all WWTPs samples after wastewater treatment. ARGs profiles varied with wastewater treatment processes, seasons and regions. This study tracked the footprint of ARGs during wastewater treatment process, which would support the assessment on the spre...Continue Reading

Citations

Jul 12, 2020·Applied and Environmental Microbiology·Shaoqing ZhangFangang Meng
May 22, 2020·Environmental Science and Pollution Research International·Jianhua ChengChen Liu
Sep 6, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Lihua LanDezhao Liu
Dec 15, 2020·Journal of Environmental Health Science & Engineering·Akram BaghaniMasoumeh Douraghi
Feb 28, 2021·Journal of Environmental Sciences (China)·Hongjie WangChangping Yu
Jul 14, 2019·The Science of the Total Environment·Yoshihiro SuzukiSatoshi Ishii
Jul 3, 2021·The Science of the Total Environment·Xinyi ShuaiHong Chen
Dec 11, 2021·The Science of the Total Environment·Yu YangTong Zhang

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