Tracking cell proliferation using a nanotechnology-based approach

Nanomedicine
Patricia Altea-ManzanoRosario M Sánchez-Martín

Abstract

To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed. [Formula: see text].

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Citations

Nov 5, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·María Victoria Cano-CortesRosario M Sanchez-Martin
Aug 21, 2018·Bioconjugate Chemistry·Teresa ValeroRosario M Sánchez Martín

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Methods Mentioned

BETA
flow
fluorescence microscopy
flow cytometry
confocal microscopy
transfect
Fluorescence
imaging technique

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