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Tracking down the sources of experimental contamination in microbiome studies

Genome Biology

Dec 17, 2014

Sophie WeissRob Knight

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Abstract

A recent report warns that DNA extraction kits and other laboratory reagents are considerable sources of contamination in microbiome experiments. The issue of contamination is particularly problematic for samples of low biomass.

Mentioned in this Paper

Extraction
DNA, Bacterial
Sequence Determinations, DNA
Tracking
DNA Contamination
Research Study
Microbiota (plant)
Microbiome
Laboratory
High-Throughput Nucleotide Sequencing
37
1
200
Paper Details
References
  • References9
  • Citations16
12

Tracking down the sources of experimental contamination in microbiome studies

Genome Biology

Dec 17, 2014

Sophie WeissRob Knight

PMID: 25608874

DOI: 10.1186/s13059-014-0564-2

Abstract

A recent report warns that DNA extraction kits and other laboratory reagents are considerable sources of contamination in microbiome experiments. The issue of contamination is particularly problematic for samples of low biomass.

Mentioned in this Paper

Extraction
DNA, Bacterial
Sequence Determinations, DNA
Tracking
DNA Contamination
37
1
200
Paper Details
References
  • References9
  • Citations16
12

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