Tracking enteric viruses in green vegetables from central Argentina: potential association with viral contamination of irrigation waters

The Science of the Total Environment
V E PrezS V Nates

Abstract

Consumption of green vegetable products is commonly viewed as a potential risk factor for infection with enteric viruses. The link between vegetable crops and fecally contaminated irrigation water establishes an environmental scenario that can result in a risk to human health. The aim of this work was to analyze the enteric viral quality in leafy green vegetables from Córdoba (Argentina) and its potential association with viral contamination of irrigation waters. During July-December 2012, vegetables were collected from peri-urban green farms (n = 19) and its corresponding urban river irrigation waters (n = 12). Also, urban sewage samples (n = 6) were collected to analyze the viral variants circulating in the community. Viruses were eluted and concentrated by polyethylene glycol precipitation and then were subject to Reverse Transcription Polymerase Chain Reaction to assess the genome presence of norovirus, rotavirus and human astrovirus. The concentrates were also inoculated in HEp-2 (Human Epidermoid carcinoma strain #2) cells to monitor the occurrence of infective enterovirus. The frequency of detection of the viral groups in sewage, irrigation water and crops was: norovirus 100%, 67% and 58%, rotavirus 100%, 75% and 5%, ast...Continue Reading

Citations

Dec 19, 2018·Food and Environmental Virology·Takayuki MiuraMichihiro Akiba
Mar 9, 2021·Environmental Science and Pollution Research International·Paulo Sérgio ScalizeLuis Rodrigo Fernandes Baumann
Dec 2, 2020·International Journal of Food Microbiology·Guadalupe Di ColaViviana E Ré
Aug 28, 2021·Food and Environmental Virology·Robertina Viviana CammarataViviana Andrea Mbayed

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