Tracking key virulence loci encoding aerobactin and salmochelin siderophore synthesis in Klebsiella pneumoniae

Genome Medicine
Margaret M C LamKathryn E Holt

Abstract

Klebsiella pneumoniae is a recognised agent of multidrug-resistant (MDR) healthcare-associated infections; however, individual strains vary in their virulence potential due to the presence of mobile accessory genes. In particular, gene clusters encoding the biosynthesis of siderophores aerobactin (iuc) and salmochelin (iro) are associated with invasive disease and are common amongst hypervirulent K. pneumoniae clones that cause severe community-associated infections such as liver abscess and pneumonia. Concerningly, iuc has also been reported in MDR strains in the hospital setting, where it was associated with increased mortality, highlighting the need to understand, detect and track the mobility of these virulence loci in the K. pneumoniae population. Here, we examined the genetic diversity, distribution and mobilisation of iuc and iro loci amongst 2503 K. pneumoniae genomes using comparative genomics approaches and developed tools for tracking them via genomic surveillance. Iro and iuc were detected at low prevalence (< 10%). Considerable genetic diversity was observed, resolving into five iro and six iuc lineages that show distinct patterns of mobilisation and dissemination in the K. pneumoniae population. The major burden o...Continue Reading

References

Feb 1, 1989·Infection and Immunity·X NassifPhilippe Sansonetti
Dec 7, 1982·Biochemistry·K KonopkaJ B Neilands
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Aug 6, 2005·Journal of Clinical Microbiology·Laure DiancourtSylvain Brisse
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael A FischbachKelly D Smith
Feb 14, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Silke I MüllerKlaus Hantke
Mar 25, 2009·Antimicrobial Agents and Chemotherapy·Alessandra Carattoli
Jul 14, 2010·Bioinformatics·Lionel GuySiv G E Andersson
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Jun 19, 2012·Future Microbiology·Alyssa S Shon, Thomas A Russo
Nov 10, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A CaponeN Petrosillo
Nov 22, 2013·The Journal of Antimicrobial Chemotherapy·Juyoun Shin, Kwan Soo Ko
Mar 20, 2014·Bioinformatics·Torsten Seemann
Apr 30, 2014·Antimicrobial Agents and Chemotherapy·Alessandra CarattoliHenrik Hasman
Oct 24, 2014·Emerging Infectious Diseases·Suzanne Bialek-DavenetSylvain Brisse
Nov 26, 2014·Genome Medicine·Michael InouyeKathryn E Holt
Jan 30, 2015·Journal of Clinical Microbiology·Virginie Passet, Sylvain Brisse
Jun 24, 2015·Bioinformatics·Ryan R WickKathryn E Holt
Jun 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kathryn E HoltNicholas R Thomson
Jul 23, 2015·MBio·Carsten StruveKaren A Krogfelt
Jul 25, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Gregg S DavisLance B Price
Mar 30, 2017·Microbial Genomics·Kelly L WyresKathryn E Holt
Apr 4, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Claire GorrieKathryn E Holt
Apr 14, 2017·Virulence·Juan Carlos Catalán-NájeraHumberto Barrios-Camacho
Jun 1, 2017·Frontiers in Cellular and Infection Microbiology·Lingling ZhanFangyou Yu
Jun 9, 2017·PLoS Computational Biology·Ryan R WickKathryn E Holt
Sep 3, 2017·The Lancet Infectious Diseases·Liang Chen, Barry N Kreiswirth
Nov 6, 2017·The Lancet Infectious Diseases·Marcus Ho Yin WongSheng Chen
Nov 6, 2017·The Lancet Infectious Diseases·Hong YaoXiang-Dang Du
Nov 6, 2017·The Lancet Infectious Diseases·Pengcheng DuChen Chen
Feb 7, 2018·Frontiers in Cellular and Infection Microbiology·Rebekah M Martin, Michael A Bachman
Apr 6, 2018·Journal of Clinical Microbiology·Ryan R WickKelly L Wyres

Citations

Feb 17, 2019·The Journal of Antimicrobial Chemotherapy·Margaret M C LamIren Høyland Löhr
Nov 4, 2019·Journal of Internal Medicine·J E ChobyD S Weiss
May 17, 2019·Clinical Microbiology Reviews·Thomas A Russo, Candace M Marr
Jul 1, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carola VenturiniJonathan R Iredell
Sep 3, 2020·International Journal of Environmental Research and Public Health·Guoying WangHongju Wang
Jun 6, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Floriana GonaMaria Lina Mezzatesta
Oct 4, 2020·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yoshifumi KubotaMasamitsu Sanui
Dec 15, 2020·International Journal of Antimicrobial Agents·Marta Hernández-GarcíaSTEP study group
Dec 29, 2020·Genome Research·Alexander Crits-ChristophJillian F Banfield
Nov 15, 2020·The Journal of Antimicrobial Chemotherapy·Vincenzo Di PilatoAR-ISS Laboratory Study Group on carbapenemase-producing Klebsiella pneumoniae
Feb 16, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Xuehuai ShenXiaomiao Hu
Mar 6, 2021·Journal of Global Antimicrobial Resistance·Peng LanYunsong Yu
May 29, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Andrew M ParrottFann Wu

Datasets Mentioned

BETA
AP006726.1
FO834905.1
CP006635.1
CP020053.1
CP010376.2
AB298504.1
CP032832
CP032834
CP010141.1

Methods Mentioned

BETA
ICE
genotyping
PCR

Related Concepts

Aerobactin
DNA Transposons
Enterobactin
Alkalescens-Dispar Group
Hydroxamic Acids
Klebsiella rhinoscleromatis
Phylogeny
Episomes
Variation (Genetics)
Pathogenicity

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

© 2021 Meta ULC. All rights reserved