Tracking KLRC2 (NKG2C)+ memory-like NK cells in SIV+ and rhCMV+ rhesus macaques

PLoS Pathogens
Daniel R RamR Keith Reeves

Abstract

Natural killer (NK) cells classically typify the nonspecific effector arm of the innate immune system, but have recently been shown to possess memory-like properties against multiple viral infections, most notably CMV. Expression of the activating receptor NKG2C is elevated on human NK cells in response to infection with CMV as well as HIV, and may delineate cells with memory and memory-like functions. A better understanding of how NKG2C+ NK cells specifically respond to these pathogens could be significantly advanced using nonhuman primate (NHP) models but, to date, it has not been possible to distinguish NKG2C from its inhibitory counterpart, NKG2A, in NHP because of unfaithful antibody cross-reactivity. Using novel RNA-based flow cytometry, we identify for the first time true memory NKG2C+ NK cells in NHP by gene expression (KLRC2), and show that these cells have elevated frequencies and diversify their functional repertoire specifically in response to rhCMV and SIV infections.

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Citations

Aug 30, 2018·Journal of Leukocyte Biology·Aimee M Beaulieu
Feb 8, 2019·Journal of Leukocyte Biology·Daniel R RamR Keith Reeves
Nov 6, 2018·Science Immunology·Chuanfeng WuCynthia E Dunbar
Aug 4, 2019·Journal of Virology·Thomas van Stigt ThansAngelique Hölzemer
Oct 28, 2019·Frontiers in Immunology·Lauren L TruittChuanfeng Wu
Feb 24, 2019·Cellular & Molecular Immunology·Xianwei WangZhigang Tian
Mar 7, 2020·The Journal of Infectious Diseases·Hunter K RoarkMark R Schleiss
Apr 10, 2020·Frontiers in Cellular and Infection Microbiology·Marc Brillantes, Aimee M Beaulieu
Mar 17, 2019·Current Opinion in Virology·Ayad AliStephen N Waggoner
May 1, 2021·International Journal of Molecular Sciences·Alessandra RossiSilvia Piconese
Jun 22, 2021·The Journal of Clinical Investigation·Gema Méndez-LagaresDennis J Hartigan-O'Connor

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Methods Mentioned

BETA
flow cytometry
density-gradient centrifugation
PMA

Software Mentioned

Flow
GraphPad Prism
RNA
GraphPad
PrimeFlow
FlowJo

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