PMID: 9420206Jan 7, 1998Paper

Tracking members of the simian immunodeficiency virus deltaB670 quasispecies population in vivo at single-cell resolution

Journal of Virology
T A ReinhartA T Haase

Abstract

Genetically distinct lentiviruses constitute a quasispecies population that can evolve in response to selective forces. To move beyond characterization of the population as a whole to the behavior of individual members, we devised an in situ hybridization approach that uses genotype-specific probes. We used probes that detect simian immunodeficiency viruses (SIV) that differ in sequence in the V1 region of the surface envelope glycoprotein (env) gene to investigate the replication and cellular tropisms of four viral variants in the tissues of infected rhesus macaques. We found that the V1 genotypic variants replicated in spatially defined patterns and to different extents at each anatomic site. The two variants that replicated most extensively in animals with AIDS were detected in both macrophages and T lymphocytes in tissues. By extension of this approach, it will be possible to investigate the role of individual lentiviruses in a quasispecies in pathogenesis and to evaluate the effects of antiviral or immunotherapeutic treatment on select members of a quasispecies.

References

Jan 1, 1990·Annual Review of Immunology·R C Desrosiers
Jun 1, 1989·Nature·V M HirschP R Johnson
Mar 1, 1994·Journal of Neuropathology and Experimental Neurology·D M RauschL E Eiden
Apr 10, 1996·AIDS Research and Human Retroviruses·M Di StefanoF Chiodi
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·C C BleulC R Mackay
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·A L EdingerR W Doms
Nov 14, 1997·The Journal of Infectious Diseases·T A ReinhartA T Haase

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Citations

Jul 2, 2003·Nature Medicine·Melissa Pope, Ashley T Haase
Jul 11, 2006·Clinical and Vaccine Immunology : CVI·Diana FinziCarl W Dieffenbach
Oct 6, 2016·PLoS Biology·Katrina A LythgoeChristophe Fraser
Apr 3, 2001·AIDS Research and Human Retroviruses·I S FortgangL S Levy
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·I M Rouzine, J M Coffin
Apr 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhi-Qiang ZhangAshley T Haase
Oct 6, 2007·Journal of Neuropathology and Experimental Neurology·Candan DepboyluEberhard Weihe

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