Tracking microevolution events among ST11 carbapenemase-producing hypervirulent Klebsiella pneumoniae outbreak strains

Emerging Microbes & Infections
Ning DongSheng Chen

Abstract

The recent convergence of genetic elements encoding phenotypic carbapenem-resistance and hypervirulence within a single Klebsiella pneumoniae host strain represents a major public concern. To obtain a better understanding of the genetic characteristic of this emerging 'superbug', the complete genomes of 3 isolates of ST11 carbapenemase-producing hypervirulent K. pneumoniae were generated using the Oxford nanopore MinION platform. Comparative whole-genome analysis identified 13 SNPs and 3 major regions of indels in the chromosomes of the clonally disseminated isolates. ISKpn18-mediated disruption in the mgrB gene, which was associated with colistin resistance, was identified in two later strains, leading to the emergence of hypervirulent K. pneumoniae that was simultaneously colistin- and carbapenem-resistant. Five plasmids were recovered from each isolate, including a 178 Kb IncHI1B/FIB-type rmpA2-bearing virulence plasmid, a 177.5 Kb IncFII/R self-transferable blaKPC-2-bearing MDR plasmid, a 99.7 Kb Incl1 plasmid and two ColRNAI-type plasmids of sizes of 11.9 and 5.6 Kb, respectively. The presence of homologous regions between the non-conjugative virulence plasmid and conjugative blaKPC-2-bearing MDR plasmid suggests that tran...Continue Reading

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Citations

May 17, 2019·Clinical Microbiology Reviews·Thomas A Russo, Candace M Marr
May 20, 2020·Journal of Clinical Laboratory Analysis·Qiang ZhaoDing-Xia Shen
Sep 29, 2020·Frontiers in Microbiology·João Alves GamaFrancisco Dionisio
Feb 10, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·May SherifAmani El-Kholy

Datasets Mentioned

BETA
CP025952

Methods Mentioned

BETA
Illumina sequencing
phosphotransferase

Related Concepts

CfiA protein, Bacteroides fragilis
Bacteriocidal Agents
Bacterial Proteins
Beta-Lactamase
Totazina
Disease Outbreaks
Klebsiella Infections
Klebsiella rhinoscleromatis
Episomes
Pathogenicity

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