Tracking Modified Vaccinia Virus Ankara in the Chicken Embryo: In Vivo Tropism and Pathogenesis of Egg Infections

Viruses
Martin C LangenmayerAsisa Volz

Abstract

The Modified Vaccinia virus Ankara (MVA) is a highly attenuated vaccinia virus serving as a promising vector vaccine platform to develop vaccines against infectious diseases. In contrast to the well-established replication deficiency and safety of MVA in mammals, much less is known about MVA infection in avian hosts. Here, we used a recombinant MVA expressing fluorescent reporter proteins under transcriptional control of specific viral early and late promoters to study in vivo tropism, distribution, and pathogenesis of MVA infections in embryonated chicken eggs. The chorioallantoic membrane (CAM) of embryonated chicken eggs was inoculated with recombinant MVA, MVA or phosphate-buffered saline. The infection was analyzed by fluorescence microscopy, histology, immunohistochemistry, and virus titration of embryonic tissues. After infection of the CAM, MVA spread to internal and external embryonic tissues with the liver as a major target organ. Macrophages and hematopoietic cells were identified as primary target cells of MVA infection and may be involved in virus spread. Increasing doses of MVA did not result in increased lesion severity or embryonic death. Despite MVA generalization to embryonic tissues, the CAM seems to be the m...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G Sutter, B Moss
Apr 1, 1974·The American Journal of Physiology·G L LinC E Cornelius
Mar 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J H Beckstead
Aug 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J H Beckstead
Apr 1, 1994·Immunology and Cell Biology·J W LowenthalJ J York
Dec 1, 1996·Pediatric Research·J van GoldeC E Blanco
Jun 5, 1998·Veterinary Immunology and Immunopathology·J MastL R Berghman
May 24, 2003·Best Practice & Research. Clinical Gastroenterology·Richard K GilroyJohn L Gollan
Nov 24, 2004·Virology Journal·Juana M Sánchez-PuigRafael Blasco
Jun 19, 2007·Poultry Science·M F Abdul-CareemS Sharif
Feb 3, 2011·Virchows Archiv : an International Journal of Pathology·Valeer J Desmet
Jun 13, 2012·Methods in Molecular Biology·Melanie KremerGerd Sutter
Jan 2, 2013·Frontiers in Bioscience (Elite Edition)·Harjit K Bhogal, Arun J Sanyal
Feb 5, 2013·Virus Research·J C Gómez-VillamandosL Carrasco
Jun 5, 2016·Veterinary Microbiology·Mariette F DucatezAsisa Volz

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Mar 30, 2020·Tissue Engineering. Part B, Reviews·Greet MerckxAnnelies Bronckaers

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