Tracking multiplex drugs and their dynamics in living cells using the label-free surface-enhanced Raman scattering technique

Molecular Pharmaceutics
Jing YangZhuyuan Wang

Abstract

Label free and real time detection of nonfluorescent drugs inside living cells has been realized by using surface-enhanced Raman scattering (SERS). For the first time, the characteristics of 6-mercapotopurine and methimazole, two different drugs, were monitored simultaneously by SERS in living cells. Particularly, the processes of diffusion and metabolism of drugs occurring in the intracellular matrix were investigated. The results indicate that the metabolism speed of 6-mercapotopurine in living HeLa cells is much faster than that of methimazole. Moreover, the detection sensitivity of intracellular drugs has also been checked and a low detection limit of 1 nM was obtained of drug 6-mercapotopurine in a single HeLa cell.

References

Dec 1, 1982·Biochemical Pharmacology·H EnglerT Nakashima
Jul 24, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Akihito NishidaTatsuji Iga
Mar 2, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Connie BrouwerJos P M Bökkerink
Sep 22, 2005·Therapeutic Drug Monitoring·John A Duley, Timothy H J Florin
Jul 21, 2006·The Journal of Physical Chemistry. B·Haifeng YangRuqin Yu
Mar 30, 2007·Talanta·Krzysztof Kuśmierek, Edward Bald
May 13, 2010·Journal of Biomedical Optics·Stefan BálintDmitri Petrov

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Citations

Feb 16, 2013·Chemical Reviews·Anthony S StenderNing Fang
Sep 25, 2018·Journal of Clinical Pharmacology·Stephen J Balevic, Michael Cohen-Wolkowiez
Jul 19, 2013·Physical Chemistry Chemical Physics : PCCP·Jun AndoSatoshi Kawata

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