Tracking of Adipose-Derived Mesenchymal Stromal/Stem Cells in a Model of Cisplatin-Induced Acute Kidney Injury: Comparison of Bioluminescence Imaging versus qRT-PCR

International Journal of Molecular Sciences
Ralf SchubertPatrick C Baer

Abstract

Determining the cell fate and the distribution of mesenchymal stromal/stem cells (MSCs) after transplantation are essential parts of characterizing the mechanisms of action and biosafety profile of stem cell therapy. Many recent studies have shown that MSCs migrate into injured tissues, but are only detectable at extremely low frequencies. We investigated the cell fate of MSCs after transplantation in an acute kidney injury (AKI) mouse model using in vivo bioluminescence imaging (BLI) and subsequent verification of cell migration using quantitative real-time polymerase chain reaction (qRT-PCR). The AKI was induced by a single injection of cisplatin (8 or 12 mg/kg). One day later, adipose-derived mesenchymal stromal/stem cells isolated from luciferase transgenic mice (Luc⁺-mASCs, 5 × 10⁵) were intravenously transplanted. Migration kinetics of the cells was monitored using BLI on day 1, 3, and 6, and finally via quantitative real-time PCR at the endpoint on day 6. Using BLI, infused Luc⁺-mASCs could only be detected in the lungs, but not in the kidneys. In contrast, PCR endpoint analysis revealed that Luc-specific mRNA could be detected in injured renal tissue; compared to the control group, the induction was 2.2-fold higher for ...Continue Reading

References

Nov 30, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·K AkagiT Shikata
Nov 1, 1995·Molecular Microbiology·C H ContagD A Benaron
Dec 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yu-An CaoChristopher H Contag
Jun 24, 2004·Journal of the American Society of Nephrology : JASN·Marina MorigiGiuseppe Remuzzi
May 11, 2006·Journal of Cell Science·Lindolfo da Silva MeirellesNance Beyer Nardi
Jul 28, 2007·Journal of the American Society of Nephrology : JASN·Baoyuan BiLloyd G Cantley
Oct 5, 2007·Annual Review of Medicine·Benjamin D Humphreys, Joseph V Bonventre
Oct 19, 2007·Journal of the American Society of Nephrology : JASN·Barbara ImbertiGiuseppe Remuzzi
May 16, 2008·American Journal of Physiology. Renal Physiology·Florian TögelChristof Westenfelder
Sep 13, 2008·Cell Stem Cell·Arnold I Caplan
Oct 22, 2008·Transplantation Proceedings·J H SungS-J Kim
Oct 14, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Fernando EzquerPaulette Conget
Feb 20, 2010·Circulation Research·John V TerrovitisEduardo Marbán
Jun 15, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Patricia E de AlmeidaJoseph C Wu
Sep 29, 2011·Nature Reviews. Clinical Oncology·Moritz F KircherJan Grimm
Dec 22, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Marina Morigi, Ariela Benigni
Apr 8, 2014·Cell Stem Cell·Patricia K NguyenJoseph C Wu
Oct 14, 2014·Tuberculosis and Respiratory Diseases·You-Sun KimYeon-Mok Oh
Nov 26, 2015·BMC Biology·Iain R Murray, Bruno Péault
Jan 13, 2016·Stem Cell Research & Therapy·Johannes Leibacher, Reinhard Henschler
May 30, 2016·Methods in Molecular Biology·Marina MorigiGiuseppe Remuzzi
Jun 13, 2018·International Journal of Molecular Sciences·Patrick C BaerHelmut Geiger

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Methods Mentioned

BETA
transfection
PCR
electrophoreses
flow cytometry
phase contrast microscopy
electrophoresis

Software Mentioned

Prism
Living Image

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