Tracking of Infused Mesenchymal Stem Cells in Injured Pulmonary Tissue in Atm-Deficient Mice.

Cells
Patrick C BaerRalf Schubert

Abstract

Pulmonary failure is the main cause of morbidity and mortality in the human chromosomal instability syndrome Ataxia-telangiectasia (A-T). Major phenotypes include recurrent respiratory tract infections and bronchiectasis, aspiration, respiratory muscle abnormalities, interstitial lung disease, and pulmonary fibrosis. At present, no effective pulmonary therapy for A-T exists. Cell therapy using adipose-derived mesenchymal stromal/stem cells (ASCs) might be a promising approach for tissue regeneration. The aim of the present project was to investigate whether ASCs migrate into the injured lung parenchyma of Atm-deficient mice as an indication of incipient tissue damage during A-T. Therefore, ASCs isolated from luciferase transgenic mice (mASCs) were intravenously transplanted into Atm-deficient and wild-type mice. Retention kinetics of the cells were monitored using in vivo bioluminescence imaging (BLI) and completed by subsequent verification using quantitative real-time polymerase chain reaction (qRT-PCR). The in vivo imaging and the qPCR results demonstrated migration accompanied by a significantly longer retention time of transplanted mASCs in the lung parenchyma of Atm-deficient mice compared to wild type mice. In conclusion...Continue Reading

References

May 11, 2006·Journal of Cell Science·Lindolfo da Silva MeirellesNance Beyer Nardi
Jun 29, 2010·Pediatric Pulmonology·Sharon A McGrath-MorrowHoward M Lederman
Jan 16, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Kenneth SinclairDaniel C Chambers
Jun 14, 2013·Pediatric Pulmonology·Scott A Schroeder, Stefan Zielen
Aug 30, 2013·Expert Opinion on Biological Therapy·Luai HuleihelMauricio Rojas
Dec 7, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jessica M OverstreetPaul J Higgins
Feb 7, 2016·The Journal of Pediatrics·Sharon A McGrath-MorrowHoward M Lederman
Oct 16, 2019·Stem Cells Translational Medicine·Alexander AveryanovVladimir Baklaushev

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Methods Mentioned

BETA
transgenic
phase contrast microscopy
PCR
electrophoresis

Software Mentioned

flexiWare
LivingImage

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