PMID: 15383583Sep 24, 2004Paper

Tracking of V beta 8.2-positive encephalitogenic T cells by complementarity-determining region 3 spectratyping and subsequent Southern blot hybridization in Lewis rats after neuroantigen sensitization

The Journal of Immunology : Official Journal of the American Association of Immunologists
Hiroshi SakumaYoh Matsumoto

Abstract

Pathogenic T cells in organ-specific autoimmune diseases use a limited number of TCR alpha- and beta-chains. In experimental autoimmune encephalomyelitis (EAE) induced in Lewis rats by immunization with myelin basic protein, encephalitogenic T cells mainly use Vbeta8.2 TCR and clonal expansion of the Vbeta8.2 spectratype containing the EAE-specific complementarity-determining region 3 (CDR3) sequence, DSSYEQYFGPG, is found in the spinal cord throughout the course of clinical EAE. In the present study we performed temporal and spatial analyses of Vbeta8.2 spectratype expansion by CDR3 spectratyping and subsequent DNA hybridization with a probe specific for the encephalitogenic CDR3 sequence to elucidate the kinetics of encephalitogenic T cells during the induction phase after neuroantigen sensitization. It was demonstrated that Vbeta8.2 spectratype expansion and/or the positive signal in Southern blot were first detected in the regional lymph nodes as early as day 3 postimmunization and was disseminated over the lymphoid organs by day 6. Because perfusion of immunized rats with PBS erased the positive signals on day 3 postimmunization, the majority of Vbeta8.2-positive encephalitogenic T cells at the very early stage would resid...Continue Reading

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Citations

Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoh MatsumotoNoritoshi Shibuya
May 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura DiluvioJoerg C Prinz
Jul 14, 2007·The Journal of Clinical Investigation·Juscilene S MenezesEli E Sercarz

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