Tracking the Response of Burkholderia cepacia G4 5223-PR1 in Aquifer Microcosms.

Applied and Environmental Microbiology
J WinklerR A Snyder

Abstract

The introduction of bacteria into the environment for bioremediation purposes (bioaugmentation) requires analysis and monitoring of microbial population dynamics to define persistence and activity from both efficacy and risk assessment perspectives. Burkholderia cepacia G4 5223-PR1 is a Tn5 insertion mutant which constitutively expresses a toluene ortho-monooxygenase that degrades trichloroethylene (TCE). This ability of G4 5223-PR1 to degrade TCE without aromatic induction may be useful for bioremediation of TCE-containing aquifers and groundwater. Thus, a simulated aquifer sediment system and groundwater microcosms were used to monitor the survival of G4 5223-PR1. The fate of G4 5223-PR1 in sediment was monitored by indirect immunofluorescence microscopy, a colony blot assay, and growth on selective medium. G4 5223-PR1 was detected immunologically by using a highly specific monoclonal antibody which reacted against the O-specific polysaccharide chain of the lipopolysaccharides of this organism. G4 5223-PR1 survived well in sterilized groundwater, although in nonsterile groundwater microcosms rapid decreases in the G4 5223-PR1 cell population were observed. Ten days after inoculation no G4 5223-PR1 cells could be detected by s...Continue Reading

References

Apr 1, 1979·Journal of General Microbiology·H Hofstra, J Dankert
Dec 1, 1992·Applied and Environmental Microbiology·M S Shields, M J Reagin
Apr 1, 1992·Journal of Clinical Microbiology·I SteinmetzD Bitter-Suermann
Mar 1, 1991·Journal of Immunological Methods·J M Luk, A A Lindberg
Mar 1, 1990·Applied and Environmental Microbiology·J M GonzalezB F Sherr
Nov 9, 1990·Science·P H Abelson
Jun 1, 1989·Applied and Environmental Microbiology·A BérubéP Payment
Sep 1, 1987·Microbiological Reviews·D B Roszak, R R Colwell
May 1, 1966·Journal of General Microbiology·R Y StanierM Doudoroff
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
May 1, 1965·The Journal of Cell Biology·J H VENABLE, R COGGESHALL
Apr 1, 1992·Applied and Environmental Microbiology·B ChristensenT Lien

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Citations

Jan 31, 2009·FEMS Microbiology Reviews·Janmejay PandeyRakesh K Jain
Apr 28, 2010·Biodegradation·Archana Chauhan, Rakesh K Jain
Jun 3, 1998·Applied and Environmental Microbiology·B B ChristensenS Molin

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