Tracking the Sources of Antibiotic Resistance Genes in an Urban Stream during Wet Weather using Shotgun Metagenomic Analyses

Environmental Science & Technology
Darshan BaralXu Li

Abstract

Stormwater runoff has been known to cause increases in bacterial loadings in urban streams. However, little is known about its impacts on antibiotic resistance genes (ARGs) in urban watersheds. This study was performed to characterize the ARG composition of various environmental compartments of an urban watershed and to quantify their contributions of microbes and ARGs to an urban stream under wet weather conditions. Shotgun metagenomic results showed that the ARG abundance in wet weather flow was significantly higher than in base flow. Multidrug resistance genes were the most common ARG type across environmental samples. Vancomycin resistance genes were abundant in embankment soil and street sweeping samples. Analyses using SourceTracker estimated storm drain outfall water to be the biggest contributor of microbes (54-57%) and ARGs (82-88%) in the urban stream during wet weather flows. Furthermore, results on street sweepings showed that wash-off from streets was the biggest known contributor of microbes (41-45%) and ARGs (92-96%) in storm drain outfall water. Pantoea and Pseudomonas were associated with the highest numbers of ARGs and were most abundant in stormwater-related samples. Results from this study can advance our kn...Continue Reading

References

Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
Jan 1, 1984·Annual Review of Microbiology·J C Barna, D H Williams
Apr 25, 2003·International Journal of Systematic and Evolutionary Microbiology·Paolo MonciardiniStefano Donadio
Jan 22, 2004·Bioinformatics·Emmanuel ParadisKorbinian Strimmer
Feb 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Thomas L Griffiths, Mark Steyvers
Feb 11, 2005·Chemical Reviews·Heinz G Floss, Tin-Wein Yu
May 17, 2006·Trends in Ecology & Evolution·Stephen R ProulxPatrick C Phillips
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Jul 18, 2006·Nature Reviews. Microbiology·Laura J V Piddock
Apr 26, 2007·Annals of Medicine·Keith Poole
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Jacek LubelskiArnold J M Driessen
Jun 7, 2008·Current Opinion in Biotechnology·Fernando BaqueroRafael Cantón
Feb 3, 2009·Chemosphere·Klaus Kümmerer
Feb 12, 2009·FEMS Microbiology Reviews·Jose Luis MartinezCarolina Alvarez-Ortega
Feb 24, 2009·Annual Review of Biochemistry·Hiroshi Nikaido
Oct 6, 2009·Journal of Environmental Management·Yong-Chul JangTimothy Townsend
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Aug 17, 2010·Bioinformatics·Robert C Edgar
Jul 19, 2011·Nature Methods·Dan KnightsScott T Kelley
Jul 27, 2011·Environmental Science & Technology·Bram SercuPatricia A Holden
Jan 17, 2012·Environmental Pollution·Young-Sik HamHayashi Nomura
Jun 8, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L GarmendiaJ L Martinez
Jan 26, 2013·The Lancet Infectious Diseases·Elizabeth M H WellingtonA Prysor Williams
Jan 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Natasha DeLeon-RodriguezKonstantinos T Konstantinidis
Mar 12, 2013·Environmental Science & Technology·Jessica J EichmillerMichael J Sadowsky
May 8, 2013·Antimicrobial Agents and Chemotherapy·Andrew G McArthurGerard D Wright
May 28, 2013·Journal of Water and Health·Suzanne YoungGregory D O'Mullan
Dec 3, 2013·Trends in Microbiology·Elisabet MartiJose Luis Balcazar
Dec 18, 2013·Environmental Science & Technology·Daqing MaoP J J Alvarez
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
May 13, 2014·Current Biology : CB·Joseph NesmePascal Simonet
Jun 16, 2014·Bioinformatics·Zuguang GuBenedikt Brors
Dec 24, 2014·Nature Reviews. Microbiology·José L MartínezFernando Baquero
Jan 7, 2016·Frontiers in Microbiology·Marco MeolaJosef Zeyer
Feb 13, 2016·Environmental Science and Pollution Research International·Songhe ZhangKwaku Anim-Larbi

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Citations

Feb 12, 2021·Environmental Science & Technology·Prakit SaingamTao Yan
May 11, 2021·The Science of the Total Environment·Yu ZhangXiaojun Shi
May 18, 2021·Access Microbiology·Kieran OsbistonLorena Teresa Fernández-Martínez
Jul 24, 2021·Environmental Science : Water Research & Technology·Eramo AlessiaN L Fahrenfeld
Jul 30, 2021·Environmental Science & Technology·Yuepeng SunXu Li
Mar 14, 2019·Environmental Science & Technology·Wenlong ZhangSiqi Fang
Aug 25, 2021·Journal of Environmental Management·Luke Vander MeerKelly N DuBois

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