Tracking the transdermal penetration pathways of optimized curcumin-loaded chitosan nanoparticles via confocal laser scanning microscopy

International Journal of Biological Macromolecules
Salma M Abdel-HafezOmaima A Sammour

Abstract

Curcumin-loaded chitosan nanoparticles intended for transdermal delivery were successfully prepared, optimized and their fate, interaction and pathway through the skin were tracked. D-optimal response surface methodology was used for the nanoparticles optimization. Xy and z-stack confocal laser scanning microscopic images were used for the particles tracking after measuring the drug permeation through the skin using Franz diffusion cells. Very small particle sizes in the range of 33.85-199.23nm accompanied with low PDI values of 0.129-0.536 of the prepared curcumin-loaded chitosan nanoparticles were obtained. TEM images revealed the spherical and non-aggregating curcumin-loaded chitosan nanoparticles. The ex-vivo permeation studies have proven the ability of the prepared chitosan nanoparticles to deliver curcumin through the skin reaching fluxes viz 5.14±1.31μgcm-2h-1. The confocal laser scanning microscopy has proven that the appendageal route is the main route of penetration of the prepared nanoparticles and has demonstrated the localization of the chitosan nanoparticles within the hair follicles from which the drug diffuses to deep layers of the skin and beyond.

Citations

Jan 11, 2020·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Juergen LademannMaxim E Darvin
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Rania M Hathout, Dina H Kassem
Apr 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Clinton GomezPranee Rojsitthisak
May 7, 2020·Advanced Pharmaceutical Bulletin·Dipak Dilip Gadade, Sanjay Sudhakar Pekamwar
Jan 12, 2019·AAPS PharmSciTech·Rajesh Sreedharan NairChee-Onn Leong
Oct 6, 2020·Macromolecular Bioscience·Anna E CaprificoGianpiero Calabrese
Oct 22, 2020·Polymers·Zoi TerzopoulouDimitrios Bikiaris
May 30, 2020·Molecular Pharmaceutics·Mingyue CuiChenjie Xu
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Subashni BhoopathyDharani Gopal
Mar 6, 2021·International Journal of Biological Macromolecules·Qiaobin Hu, Yangchao Luo
Mar 20, 2021·International Journal of Biological Macromolecules·Serag Eldin I ElbehairiHani S Hafez
May 1, 2021·Pharmaceutics·Andreea Teodora IacobLenuta Profire
Apr 24, 2021·Drug Discovery Today·Anna E CaprificoGianpiero Calabrese
Apr 6, 2018·Journal of Agricultural and Food Chemistry·Gang LiuXinguang Qin
Feb 6, 2020·ACS Omega·Rania M HathoutJohn G Hardy

❮ Previous
Next ❯

Related Concepts