Trading polymeric microspheres: exchanging DNA molecules via microsphere interaction

Colloids and Surfaces. B, Biointerfaces
Nobuyuki MorimotoMakoto Suzuki

Abstract

A new class of artificial molecular transport system is constructed by polymeric microspheres. The microspheres are prepared by self-assembly of poly(ethylene glycol)-block-poly(3-dimethyl(methacryloyloxyethyl)ammonium propane sulfonate), PEG-b-PDMAPS, by intermolecular dipole-dipole interaction of sulfobetaine side chains in water. Below the upper critical solution temperature (UCST) of PEG-b-PDMAPS, the microspheres (∼1μm) interact with other microspheres by partial and transit fusion. In order to apply the interaction between microspheres, a 3'-TAMRA-labeled single-stranded DNA oligomer (ssDNA) is encapsulated into a PEG-b-PDMAPS microsphere by thermal treatment. The exchange of ssDNA between microspheres is confirmed by fluorescence resonance energy transfer (FRET) quenching derived from double-stranded formation with complementary 5'-BHQ-2-labeled ssDNA encapsulated in PEG-b-PDMAPS microspheres. The exchange rate of ssDNA is controllable by tuning the composition of the polymer. The contact-dependent transport of molecules can be applied in the areas of microreactors, sensor devices, etc.

References

Aug 10, 2002·Science·Dennis E Discher, Adi Eisenberg
Apr 11, 2006·Biomacromolecules·George S GeorgievIvo A Ivanov
Jun 22, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·C J F RijckenC F van Nostrum
Sep 11, 2012·Macromolecular Rapid Communications·Jan Seuring, Seema Agarwal
Dec 7, 2013·Macromolecular Rapid Communications·Nobuyuki MorimotoMakoto Suzuki

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Citations

Nov 21, 2015·Chemical Communications : Chem Comm·Lianjiang TanZhifeng Shao
May 3, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Kohei IritaniYoshito Tobe

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