TRAF4, a new substrate of SIAH1, participates in chemotherapy resistance of breast cancer cell by counteracting SIAH1-mediated downregulation of β-catenin

Breast Cancer Research and Treatment
Hua-Yan RenHuixiang Li

Abstract

TRAF4 plays an important role in the development and progression of breast cancer, but its impact on chemotherapy resistance is as yet, however, poorly understood. Western blotting, immunoprecipitation, and immunofluorescence staining were used to identify and verify that TRAF4 was a novel substrate of SIAH1 and prevented SIAH1-mediated β-catenin degradation. Cell proliferation analysis and Flow cytometry analysis were utilized to detect TRAF4's function on the growth-inhibitory effect of etoposide. Immunohistochemistry was used to detect the expression of TRAF4, SIAH1, and β-catenin. Statistical analysis was used to analyze the relationships between them with clinical parameters and curative effect of chemotherapy pathologically. Our results suggested that TRAF4 prevents SIAH1-mediated β-catenin degradation. TRAF4 was a novel substrate of SIAH1 and the TRAF domain of TRAF4 was critical for binding to SIAH1. TRAF4 reduced the growth-inhibitory effect of etoposide via reducing the number of S-phase cells and suppressing cell apoptosis. Concordantly, we found that breast cancer patients with a low-TRAF4 expression benefited most from chemotherapy, who had higher tumor volume reduction rate and better pathological response, while,...Continue Reading

References

Oct 19, 2001·Oncogene·J R Bradley, J S Pober
Dec 14, 2001·Nature Structural Biology·Galina PolekhinaDavid D L Bowtell
Nov 2, 2002·The EMBO Journal·Hasem HabelhahZe'ev Ronai
May 28, 2004·Human Molecular Genetics·Julian P VenablesDavid J Elliott
Dec 8, 2004·Cellular and Molecular Life Sciences : CMLS·E M Esparza, R H Arch
Jun 27, 2006·Oncogene·S Camilleri-BroëtC H Régnier
Aug 5, 2006·Journal of Cellular Biochemistry·Robert J NadeauRobert Friesel
Sep 14, 2006·Cancer Biology & Therapy·Laura M Rozan, Wafik S El-Deiry
Feb 20, 2007·Trends in Cell Biology·Axel MogkBernd Bukau
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Aug 29, 2009·Science·Wei-Lei YangHui-Kuan Lin
Nov 19, 2009·Cancer Research·Colin M HouseDavid D L Bowtell
Feb 26, 2010·The Journal of Biological Chemistry·Yoana N DimitrovaWalter J Chazin
May 18, 2010·Biochemical and Biophysical Research Communications·Huiling WuKeke Huo
Jul 8, 2011·Cell Cycle·Yoshito NaganoShu-ichi Matsuzawa
Mar 6, 2012·Journal of Cell Science·Meredith B MetzgerAllan M Weissman
Jun 12, 2012·Cell·Hans Clevers, Roel Nusse
Oct 6, 2012·Leukemia·O H KrämerS K Knauer
Aug 27, 2013·Molecular Cell·Long ZhangPeter Ten Dijke
Aug 29, 2013·Protein & Cell·Fengfeng NiuZhi-Jie Liu
Oct 25, 2013·Cancer Research·Wei LiZigang Dong
Jan 15, 2014·Acta Crystallographica. Section D, Biological Crystallography·Jong Hwan YoonHyun Ho Park
May 30, 2014·The Lancet Oncology·Lei FanPaul E Goss
Jun 10, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jessica LawrenceDavid Argyle
Jan 31, 2017·The Journal of Investigative Dermatology·Hiroyuki YamamotoZigang Dong
Apr 26, 2017·World Journal of Clinical Oncology·Viviana Masoud, Gilles Pagès

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis