TRAF6 is required for BLyS-mediated NF-κB signaling in multiple myeloma cells

Medical Oncology
Xinfeng WangHong-ming Huang

Abstract

Tumor necrosis factor receptor-associated factor 6 (TRAF6) transduces signals from members of the IL-1R/TLR and TNFR superfamilies to the transcription factors NF-κB and AP1. Elevated expression of the TNF family member B-lymphocyte stimulator (BLyS) in multiple myeloma (MM) has been described recently. However, the precise process by which BLyS signals in myeloma cell remains unknown. Here, we identified increased expression of TRAF6 in MM patient cells and the MM cell lines U266, RPMI8226, and KM3. Furthermore, rhBLyS induced TRAF6 up-regulation in these cells in a dose-dependent manner. Both the classical and alternative NF-κB pathways were activated by rhBLyS treatment. Depletion of TRAF6 by siRNA decreased levels of p-p65 and p-p100, even after stimulation with rhBLyS. Down-regulation of TRAF6 also abrogated rhBLyS-mediated cell viability. These findings suggest that TRAF6 is required for BLyS-mediated NF-κB signaling in myeloma cells and is a potential molecular therapeutic target in MM.

References

Jan 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marcel BattenFabienne Mackay
Mar 10, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Takaomi SandaTakashi Okamoto
Oct 6, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Teru HideshimaKenneth C Anderson
Jun 5, 2007·British Journal of Haematology·Michel JourdanBernard Klein
Jun 5, 2007·Biochemical and Biophysical Research Communications·Ann T PoblenzBryant G Darnay
Nov 26, 2008·Clinical Biochemistry·Shaoqing JuRenqian Zhong
Feb 4, 2009·European Journal of Haematology·Pu JiangShaoqing Ju
Oct 5, 2010·Oncotarget·Yulia N Demchenko, W Michael Kuehl
Sep 14, 2011·The Journal of Clinical Investigation·Daniel T StarczynowskiAly Karsan
Mar 14, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Dror Ben-DavidAbraham Z Reznick
Mar 24, 2012·Clinical Lymphoma, Myeloma & Leukemia·Hong LiuBenjamin Bonavida
Oct 12, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lou ZhongQingsheng You
Jan 2, 2013·Frontiers in Bioscience (Landmark Edition)·Ping WangDongqing Zhang
Jun 14, 2013·Journal of Molecular Signaling·Ping Xie
Nov 6, 2014·The Journal of Biological Chemistry·Sae Mi WiKi-Young Lee

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.