PMID: 11920827Mar 29, 2002Paper

Trafficking mechanism of West Nile (Sarafend) virus structural proteins

Journal of Medical Virology
J J H Chu, M L Ng

Abstract

Previous studies have shown that West Nile (Sarafend) virus matured by budding at the plasma membrane, which differs from the usual intracellular maturation of other flaviviruses. The present study investigated the trafficking mechanism of the envelope (E) and capsid (C) proteins of West Nile (Sarafend) virus during the replication cycle. The use of time-based double-immunofluorescence labelling coupled with the Triton X-100 extraction procedure revealed that both the E and C proteins were transported from the perinuclear region towards the plasma membrane along the microtubules simultaneously. The strong association of these virus proteins with the microtubules was demonstrated further with Triton X-100 extraction procedure coupled with double immunogold-labelling. Extraction of infected cells with Triton X-100 in high salt also revealed that virus E proteins were associated with the microtubules via protein-protein interaction. The disruption of microtubules with vinblastine sulphate inhibited the trafficking of both the virus E and C proteins. Both virus structural proteins were observed to co-localise and retained within vinblastine sulphate-induced microtubulin paracrystals. Extracellular virus production was also reduced ...Continue Reading

Citations

Jan 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Iris AlroyYuval Reiss
Jun 23, 2004·Biochemical and Biophysical Research Communications·Hui-Yee Chee, Sazaly AbuBakar
Feb 7, 2019·Virologica Sinica·Yue ZhangYaming Jiu

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