PMID: 9421932Jan 9, 1998Paper

Trafficking of wheat gluten proteins in transgenic tobacco plants: gamma-gliadin does not contain an endoplasmic reticulum-retention signal

Planta
J A NapierPeter R Shewry

Abstract

Wild-type and mutated forms of the wheat (Triticum aestivum L.) storage protein gamma-gliadin were expressed in transgenic tobacco (Nicotiana tabacum L. cv. NVS) under the control of the 35S cauliflower mosaic virus (CaMV) promoter in order to determine what, if any, endogenous targeting signals are present in the wild-type gamma-gliadin protein. The mutant forms of the protein were modified by the addition of a KDEL or HDEL C-terminal endoplasmic reticulum-retention signal, or the addition of a C-terminal propeptide from barley lectin which has been shown to be necessary and sufficient for targeting to the vacuole. Only modified forms of the protein accumulated in leaves of transgenic tobacco, although the transcript levels were similar for all the constructs. Pulse-chase analysis indicated that whereas the wild-type gamma-gliadin was rapidly turned over in tobacco leaves, KDEL and HDEL forms were highly stable. The vacuolar-signal mutant protein accumulated in tobacco leaves, but migrated on sodium dodecyl sulphate-polyacrylamide gel electrophoresis with a lower mobility than wild-type gamma-gliadin, due in part to glycosylation of the C-terminal propeptide. The vacuolar-signal mutant protein was turned over slowly in tobacco...Continue Reading

Citations

Oct 21, 2010·Functional & Integrative Genomics·Maoyun SheWujun Ma
Jul 19, 2005·Transgenic Research·Ming-Li WangHenrik H Albert
May 14, 1999·Trends in Plant Science·A Vitale, N V Raikhel
Mar 6, 1999·Current Opinion in Plant Biology·K Matsuoka, S Y Bednarek
Jun 10, 2008·Journal of Experimental Botany·Maddalena de VirgilioAlessandro Vitale
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Peter R ShewryArthur S Tatham
Nov 13, 2007·Journal of Food Science·Faqir Muhammad AnjumMuhammad Umair Arshad
Feb 11, 2015·Plant Biotechnology Journal·Xiaodong SunAlan Bennett
Aug 20, 2016·Frontiers in Plant Science·Emanuela PedrazziniAlessandro Vitale

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