TRAIL-R3-related apoptosis: epigenetic and expression analyses in women with ovarian neoplasia

Gynecologic Oncology
Letícia da Conceição BragaAgnaldo Lopes da Silva-Filho

Abstract

To assess the expression of TRAIL-R3 and the methylation of a CpG island within the TRAIL-R3 promoter both in cystadenoma tumors and primary and metastatic epithelial ovarian carcinoma (EOC). RNA was obtained from women with normal ovarian (NO) tissues (n=18), ovarian serous cystadenoma tumors (n=11) and EOC (n=16) using Trizol. Quantitative PCR (qRT-PCR) was performed to quantify the relative levels of TRAIL-R3. The methylation frequency of the CpG island in the TRAIL-R3 promoter was assessed using the methylation-specific PCR (MSP) assay after DNA bisulfite conversion. The differences between the groups were evaluated using the chi-square, Student's t, ANOVA, Mann-Whitney U, Wilcoxon or Kruskal-Wallis tests as indicated. The survival rates were calculated using the Kaplan-Meier method. Cystadenoma and metastatic EOC tumors expressed significantly more TRAIL-R3 mRNA than primary EOC tumors. Methylation of the TRAIL-R3 promoter was absent in NO tissues, while hemimethylation of the TRAIL-R3 promoter was frequently found in the neoplasia samples with 45.4% of the cystadenoma tumors, 8.3% of the primary EOC samples and 11.1% of the metastatic EOC samples showing at least partial methylation (p=0.018). Neither the expression of TR...Continue Reading

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Citations

Jan 15, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Letícia da Conceição BragaAgnaldo Lopes da Silva Filho
Nov 6, 2013·Archives of Gynecology and Obstetrics·Letícia da Conceição BragaAgnaldo Lopes da Silva Filho
Jan 8, 2018·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Aline Brito de LimaLetícia da Conceição Braga
Dec 4, 2020·International Journal of Molecular Sciences·Agnieszka SzyposzynskaAleksandra Klimczak

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