Trans-acting untranslated elements of groundnut rosette virus satellite RNA are involved in symptom production

The Journal of General Virology
M E Taliansky, D J Robinson

Abstract

Isolates of groundnut rosette umbravirus (GRV) contain a satellite RNA (sat-RNA), about 900 nucleotides (nt) in length, different variants of which are responsible for the symptoms of different forms of rosette disease in groundnuts and, in the particular instance of sat-RNA YB3b, for the production of yellow blotch symptoms in Nicotiana benthamiana. Sat-RNA YB3b does not affect the accumulation of GRV genomic or subgenomic RNAs in infected plants. Replication of sat-RNA YB3b and induction of yellow blotch symptoms do not require the production of any sat-RNA-encoded proteins. Experiments with deletion mutants identified three functional untranslated elements in sat-RNA YB3b. One (designated R) comprises nt 47-281, is essential for sat-RNA replication and appears to be cis-acting. The other two (designated A and B) comprise nt 280-470 and 629-849, respectively, are both involved in yellow blotch symptom production and can act in trans. Element A contains the determinant that is unique to sat-RNA YB3b. The process of symptom induction by sat-RNA YB3b apparently involves a novel type of specific interaction of two untranslated RNA elements, which can complement each other, with a host factor or factors.

Citations

Nov 10, 2015·Molecular Plant-microbe Interactions : MPMI·Peter Palukaitis
Jul 31, 2004·Annual Review of Phytopathology·Anne E SimonZoltán Havelda
Oct 24, 2008·Phytopathology·C M DeomP Subrahmanyam
Jul 18, 2003·The Journal of General Virology·Michael E Taliansky, David J Robinson
Dec 7, 2007·The Plant Cell·Maria-Elena RodioFrancesco Di Serio

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