Abstract
TRPV1, a ligand-gated ion channel expressed in nociceptive sensory neurons is modulated by a variety of intracellular signaling pathways. Dopamine is a neurotransmitter that plays important roles in motor control, cognition, and pain modulation in the CNS, and acts via a variety of dopamine receptors (D1R-D5R), a class of GPCRs. Although nociceptive sensory neurons express D1-like receptors, very little is known about the effect of dopamine on TRPV1 in the peripheral nervous system. Therefore, in this study, we examined the effects of D1R activation on TRPV1 in mouse DRG neurons using Ca(2+) imaging and immunohistochemical analysis. The D1R agonist SKF-38393 induced reproducible Ca(2+) responses via Ca(2+) influx through TRPV1 rather than Ca(2+) mobilization from intracellular Ca(2+) stores. Immunohistochemical analysis revealed co-expression of D1R and TRPV1 in mouse DRG neurons. The PLC-specific inhibitor blocked the SKF-38393-induced Ca(2+) response, whereas the PKC, DAG lipase, AC, and PKA inhibitors had no effect on the SKF-38393-induced Ca(2+) response. Taken together, our results suggest that the SKF-38393-induced Ca(2+) response results from the direct activation of TRPV1 by a PLC/DAG-mediated membrane-delimited pathway...Continue Reading
References
Sep 9, 1985·Brain Research·G Skagerberg, O Lindvall
Jan 20, 1983·Nature·J Price, A W Mudge
Jan 1, 1983·Neuroscience and Biobehavioral Reviews·J N Joyce
Jan 1, 1995·Medical Hypotheses·C N RamchandG P Hemmings
Jan 1, 1993·Brain Research Bulletin·E PhilippeF Gaulin
Jul 27, 2001·Journal of Chemical Neuroanatomy·Y L HurdG C Sedvall
Aug 27, 2002·Neuron·Gautam BhaveRobert W Gereau
Apr 5, 2003·Journal of Neurochemistry·Li-Qing JinEitan Friedman
Dec 5, 2003·Nature·David E Clapham
Jul 28, 2004·Pain·Takao ShimizuMasahiro Nomoto
Sep 24, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zhi-Ye ZhuangRu-Rong Ji
May 5, 2005·Canadian Journal of Physiology and Pharmacology·Guo-Hua LiZhi-Wang Li
Aug 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Diana M BautistaPeter M Zygmunt
Mar 7, 2006·Experimental Neurology·Pablo BrumovskyTomas Hökfelt
Mar 21, 2006·Journal of Chemical Neuroanatomy·Pablo BrumovskyTomas Hökfelt
Jan 30, 2008·The Journal of Pain : Official Journal of the American Pain Society·Hyun Yeong KimSeog Bae Oh
Oct 2, 2008·Molecular Pain·Dong Ho WooC Justin Lee
Aug 14, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yong Ho KimSeog Bae Oh
Apr 28, 2010·Brain Research·Hong-Yuan ChuXuechu Zhen
Oct 23, 2010·PloS One·Quentin BarraudImad Ghorayeb
Oct 29, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kiyomitsu ShoudaiMichael C Andresen
Nov 6, 2010·Pain·Wataru TaniguchiMunehito Yoshida
Aug 4, 2012·Neuroscience·P R BrumovskyG F Gebhart
Jul 12, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Viktor LukacsTibor Rohacs
Nov 29, 2013·Molecular Pain·William GalbavyMichelino Puopolo
Dec 7, 2013·PloS One·Peter M ZygmuntEdward D Högestätt
Citations
Oct 8, 2016·Journal of Neuroscience Research·Peng LiuYong-Hui Dang
Aug 23, 2019·Journal of Psychopharmacology·In-Jee YouChoon-Gon Jang
Feb 15, 2019·Neural Regeneration Research·Michelino Puopolo
Aug 25, 2017·Scientific Reports·Kentaro OnoBrian L Schmidt
Aug 25, 2020·ACS Chemical Neuroscience·Adithya MohandassBaskaran Thyagarajan