Trans-Corneal Subretinal Injection in Mice and Its Effect on the Function and Morphology of the Retina

PloS One
Yan QiJijing Pang

Abstract

To introduce a practical method of subretinal injection in mice and evaluate injection-induced retinal detachment (RD) and damage using a dynamic imaging system, electrophysiology, and histology. After full dilation of a 2-month-old C57BL/6J mouse pupil, the cornea near the limbus was punctured with a 30 ½-gague disposable beveled needle. A 33 ½-gauge blunt needle was inserted through the corneal perforation into the anterior chamber, avoiding the lens before going deeper into the vitreous cavity, and penetrating the inner retina to reach the subretinal space. The mice were divided into four groups: in group 1, about 80-100% of the retina was filled with subretinally injected solution; in group 2, approximately 50-70% of the retina was filled with injected solution; in group 3, the procedures were stopped before solution injection; and non-injected eyes were used as the negative control in group 4. An optical coherence tomography (OCT) imaging system was used to monitor retinal reattachment during the first three days following the injections. Histological and functional changes were examined by light microscopy and electroretinography (ERG) at five weeks post-injection. After a short-term training, a 70% success rate with 50% ...Continue Reading

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Citations

Dec 4, 2016·Advanced Drug Delivery Reviews·Jayoung KimJordan J Green
Apr 21, 2019·Stem Cells Translational Medicine·Brittni A ScruggsElliott H Sohn
Mar 20, 2020·Pharmaceutics·Rubén Varela-FernándezFrancisco J Otero-Espinar
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Dec 4, 2020·The Application of Clinical Genetics·Srikanta Kumar PadhySubhadra Jalali
Jan 21, 2021·Pharmaceutics·Hyeong Min Kim, Se Joon Woo

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Methods Mentioned

BETA
transfect
dissecting
light microscopy

Clinical Trials Mentioned

NCT00481546

Software Mentioned

SPSS

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