Trans-dominant cellular inhibition of DC-SIGN-mediated HIV-1 transmission

Retrovirology
Li WuVineet N KewalRamani

Abstract

Dendritic cell (DC) transmission of human immunodeficiency virus (HIV) to CD4+ T cells occurs across a point of cell-cell contact referred to as the infectious synapse. The relationship between the infectious synapse and the classically defined immunological synapse is not currently understood. We have recently demonstrated that human B cells expressing exogenous DC-SIGN, DC-specific intercellular adhesion molecule-3 (ICAM-3)-grabbing nonintegrin, efficiently transmit captured HIV type 1 (HIV-1) to CD4+ T cells. K562, another human cell line of hematopoietic origin that has been extensively used in functional analyses of DC-SIGN and related molecules, lacks the principal molecules involved in the formation of immunological synaptic junctions, namely major histocompatibility complex (MHC) class II molecules and leukocyte function-associated antigen-1 (LFA-1). We thus examined whether K562 erythroleukemic cells could recapitulate efficient DC-SIGN-mediated HIV-1 transmission (DMHT). Here we demonstrate that DMHT requires cell-cell contact. Despite similar expression of functional DC-SIGN, K562/DC-SIGN cells were inefficient in the transmission of HIV-1 to CD4+ T cells when compared with Raji/DC-SIGN cells. Expression of MHC class...Continue Reading

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Citations

Oct 26, 2006·Nature Reviews. Immunology·Li Wu, Vineet N KewalRamani
Oct 11, 2005·Viral Immunology·Caroline GilbertMichel J Tremblay
May 4, 2007·Journal of Virology·Nuria Izquierdo-UserosJavier Martinez-Picado
Sep 12, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thijs van MontfortWilliam A Paxton
Nov 12, 2019·Frontiers in Immunology·Alba Martín-Moreno, Mª Angeles Muñoz-Fernández

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Methods Mentioned

BETA
transfection
fluorescence-activated cell sorting
FACS
ELISA
flow cytometry
enzyme-linked immunosorbent assay

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