Trans effects on cysteine ligation in the proximal His93Cys variant of horse heart myoglobin

Biochemistry
D P HildebrandA G Mauk

Abstract

Three variants of horse heart myoglobin (Mb) in which the proximal His93 residue has been replaced with a Cys residue have been constructed and studied by NMR, EPR, and MCD spectroscopy to evaluate the contributions of proximal and distal residues to the coordination environment of the heme iron in these proteins. Although no experimental conditions were identified that allowed quantitative ligation of the cysteine residue to the heme iron in the His93Cys variant, all of the spectroscopic evidence collected for the His93Cys/His64Ile and His93Cys/His64Val double variants supports the assignment of thiolate as the ligand to iron in the oxidized forms of these variants. The double metMb variants exhibit Soret maxima that are considerably blue-shifted, 1H NMR spectra with decreased mean methyl resonances, and EPR spectra with highly rhombic g values. These spectroscopic data for the Fe(III) variants resemble the corresponding properties reported for ferricytochrome P-450. The decrease in the reduction potential of the double variants by 280 mV relative to wild-type protein is also consistent with the low midpoint potential of cytochrome P-450. MCD spectroscopy of these variants confirms that the proximal cysteine residue is not bou...Continue Reading

Citations

Oct 17, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Stefan W VetterDavid B Goodin
Sep 19, 2002·Journal of Inorganic Biochemistry·François OgliaroSason Shaik
Nov 10, 2011·Biochemistry·Brian San FranciscoRobert G Kranz
Jul 3, 2007·Biochemistry·Yury KamenskyGraham Palmer
Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shangwei HouToshinori Hoshi
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Roshan PereraJohn H Dawson
Jul 23, 2009·Archives of Biochemistry and Biophysics·Enrica DroghettiGiulietta Smulevich
Apr 7, 2009·Current Opinion in Chemical Biology·Michael T Green
Aug 11, 2006·Angewandte Chemie·Yi Lu
Apr 1, 2006·Biochemical and Biophysical Research Communications·Jj L Miranda
Oct 3, 2003·Archives of Biochemistry and Biophysics·Neesha PatelEmma Lloyd Raven
Dec 3, 1999·Methods : a Companion to Methods in Enzymology·V S Sharma, D Magde
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brian San FranciscoRobert G Kranz
Jan 17, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Patrik RydbergUlf Ryde
May 25, 2005·Chembiochem : a European Journal of Chemical Biology·Rajamanickam Murugan, Shyamalava Mazumdar
Jun 9, 2005·Chemical Reviews·Ilia G DenisovIlme Schlichting
Jul 18, 2001·Journal of the American Chemical Society·T UnoA J Wilkinson

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