Trans-Graft Protection Against Pierce's Disease Mediated by Transgenic Grapevine Rootstocks

Frontiers in Plant Science
Abhaya M DandekarPaulo A Zaini

Abstract

A field study showed that transgenic grapevine rootstocks can provide trans-graft-mediated protection to a wild type scion against Pierce's disease (PD) development. We individually field-tested two distinct strategies. The first expressed a chimeric antimicrobial protein (CAP) that targeted the functionality of the lipopolysaccharide (LPS) surface of Xylella fastidiosa (Xf), the causative agent of PD. The second expressed a plant polygalacturonase inhibitory protein (PGIP) that prevents PD by inhibiting breakdown of pectin present in primary cell walls. Both proteins are secreted to the apoplast and then into the xylem, where they migrate past the graft union, transiting into the xylem of the grafted scion. Transgenic Vitis vinifera cv. Thompson Seedless (TS) expressing ether CAP or PGIP were tested in the greenhouse and those lines that showed resistance to PD were grafted with wild type TS scions. Grafted grapevines were introduced into the field and tested over 7 years. Here we present data on the field evaluation of trans-graft protection using four CAP and four PGIP independent rootstock lines, compared to an untransformed rootstock. There was 30 to 95% reduction in vine mortality among CAP- and PGIP-expressing lines afte...Continue Reading

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Citations

May 15, 2019·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Stephen Parnell
Apr 4, 2021·International Journal of Molecular Sciences·Manuel RubioPedro Martínez-Gómez
Jun 22, 2021·Frontiers in Plant Science·Arya Bagus Boedi IswantoSang Hee Kim
Jul 19, 2021·Horticulture Research·Maria Lobato-GómezPatricia Sarai Girón-Calva
Nov 28, 2021·Plants·Kourosh VahdatiShawn A Mehlenbacher

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Methods Mentioned

BETA
PCR
transgenic
genetic modification

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