Trans-kingdom transposition of the Drosophila element mariner within the protozoan Leishmania

Science
F J Gueiros-Filho, S M Beverley

Abstract

Transposable elements of the mariner/Tc1 family are postulated to have spread by horizontal transfer and be relatively independent of host-specific factors. This was tested by introducing the Drosophila mauritiana element mariner into the human parasite Leishmania major, a trypanosomatid protozoan belonging to one of the most ancient eukaryotic lineages. Transposition in Leishmania was efficient, occurring in more than 20 percent of random transfectants, and proceeded by the same mechanism as in Drosophila. Insertional inactivation of a specific gene was obtained, and a modified mariner element was used to select for gene fusions, establishing mariner as a powerful genetic tool for Leishmania and other organisms. These experiments demonstrate the evolutionary range of mariner transposition in vivo and underscore the ability of this ubiquitous DNA to parasitize the eukaryotic genome.

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A CruzS M Beverley
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F SchwartzR Kucherlapati
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J H LeBowitzS M Beverley
Jun 29, 1990·Cell·N Agabian
Nov 8, 1990·Nature·A Cruz, S M Beverley
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M L SoginJ H Gunderson
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Nov 15, 1995·Molecular & General Genetics : MGG·C J CoatesP W Atkinson
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·T G LoukerisC Savakis
Nov 1, 1993·Molecular and Biochemical Parasitology·D J Freedman, S M Beverley

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Citations

Apr 3, 2007·Applied Microbiology and Biotechnology·Markus MeissnerWilliam J Sullivan
May 8, 2003·Molecular Genetics and Genomics : MGG·O LadendorfJ Kämper
Sep 24, 2009·Molecular Genetics and Genomics : MGG·Sophie CasteretFlorence Rouleux-Bonnin
Sep 19, 2009·Genetica·Jeziel D DamascenoLuiz R O Tosi
Oct 23, 2009·Genetica·Xavier ThomasSylvaine Renault
May 4, 2001·International Journal for Parasitology·G A Cross
Sep 10, 1999·International Journal for Parasitology·J G WaterkeynA F Cowman
Feb 13, 2001·Molecular and Biochemical Parasitology·S YanK Stuart
May 3, 2002·Molecular and Biochemical Parasitology·Huafang ShiElisabetta Ullu
May 14, 2003·Molecular and Biochemical Parasitology·Kelly A Robinson, Stephen M Beverley
Dec 10, 1999·Molecular and Biochemical Parasitology·C LemleyK D Stuart
Mar 16, 2000·Molecular and Biochemical Parasitology·S AntoniaziA K Cruz
Aug 4, 1999·Trends in Genetics : TIG·R H PlasterkZ Ivics
Jun 15, 1999·Parasitology Today·A C Ivens, J M Blackwell
Aug 26, 1999·Parasitology Today·C E Clayton
Nov 26, 1999·Gene·D Kordis, F Gubensek
Jul 17, 1998·Current Opinion in Structural Biology·J Jurka
Feb 13, 2001·Insect Biochemistry and Molecular Biology·A M Handler
Aug 14, 2003·Insect Biochemistry and Molecular Biology·Raymond WilsonDavid A O'Brochta
Feb 5, 2002·Trends in Microbiology·V Magrini, W E Goldman
Dec 2, 2000·Microbes and Infection·S Bowman, P Horrocks
Apr 5, 2013·Parasitology·Sam AlsfordDavid Horn
Feb 3, 1998·Nature Biotechnology·A Dawson, D J Finnegan
Jan 2, 2003·Nature Reviews. Genetics·Stephen M Beverley
Sep 17, 2004·Molecular Biology and Evolution·Joana C SilvaJane M Carlton
May 16, 2009·Molecular Biology and Evolution·Eugene A Gladyshev, Irina R Arkhipova
Jun 5, 1998·Nucleic Acids Research·G J SchoutenR H Plasterk
Sep 15, 2001·Molecular Biology and Evolution·V ZupunskiD Kordis
Feb 13, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stephen M BeverleyYujia Wang
Apr 16, 2003·Genome Research·Irene CastanoBrendan P Cormack
Jan 23, 1999·Immunological Reviews·J J MarchalonisV S Hohman
Oct 7, 2004·Applied and Environmental Microbiology·Philip E StewartPatricia A Rosa
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Bill WicksteadKeith Gull

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