trans-[Ru(NO)(NH3)4(py)](BF4)3.H2O encapsulated in PLGA microparticles for delivery of nitric oxide to B16-F10 cells: Cytotoxicity and phototoxicity

Journal of Inorganic Biochemistry
Anderson J GomesElia Tfouni

Abstract

The NO donor trans-[Ru(NO)(NH(3))(4)(py)](BF(4))(3).H(2)O (py=pyridine) was loaded into poly-lactic-co-glycolic acid (PLGA) microparticles using the double emulsification technique. Scanning electron microscopy (SEM) and dynamic light scattering revealed that the particles are spherical in shape, have a diameter of 1600nm, and have low tendency to aggregate. The entrapment efficiency was 25%. SEM analysis of the melanoma cell B16-F10 in the presence of the microparticles containing the complex trans-[Ru(NO)(NH(3))(4)(py)](BF(4))(3).H(2)O (pyMP) showed that the microparticles were adhered to the cell surface after 2h of incubation. The complex with concentrations lower than 1x10(-4)M did not show toxicity in B16-F10 murine cells. The complex in solution is toxic at higher concentrations (>1x10(-3)M), with cell death attributed to NO release following the reduction of the complex. pyMP is not cytotoxic due to the lower bioavailability and availability of the entrapped complex to the medium and its reducing agents. However, pyMP is phototoxic upon light irradiation. The phototoxicity strongly suggests that cell death is due to NO release from trans-[Ru(NO)(NH(3))(4)(py)](3+). This work shows that pyMP can serve as a model for a dr...Continue Reading

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Citations

Jan 20, 2009·The Journal of Antimicrobial Chemotherapy·André C AmaralMaria Sueli S Felipe
Dec 20, 2011·Nitric Oxide : Biology and Chemistry·Elia TfouniDouglas Wagner Franco
Oct 28, 2008·Nitric Oxide : Biology and Chemistry·Cezar R PestanaCarlos Curti
Jan 25, 2017·Expert Opinion on Drug Delivery·Mahmoud A ElnaggarYoon Ki Joung
Dec 22, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Alexis D Ostrowski, Peter C Ford
Jun 18, 2010·Chemical Society Reviews·Salvatore Sortino
Aug 19, 2010·Chemical Communications : Chem Comm·Daniel CrespyAlexander Schiller
Jan 29, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Mithun RoyAkhil R Chakravarty
Nov 9, 2012·Small·Noora NaghaviAlexander M Seifalian

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