Trans-targeting of protease substrates by conformationally activating a regulable ClpX-recognition motif

Molecular Microbiology
Kimberly R Marshall-Batty, H Nakai

Abstract

Conversion of bacteriophage Mu repressor to ClpXP-sensitive form correlates with induced local flexibility at the ClpX recognition motif located at the C-terminal end. Changing the C-terminal valine to an alanine (RepV196A) caused the degradation tag to be constitutively active like that of mutant repressors called Vir, which have a dominant ClpXP-sensitive conformation. However, unlike Vir, RepV196A was unable to convert wild-type repressor (Rep) to the ClpXP-sensitive form. In mixtures with Rep, only RepV196A was rapidly degraded by ClpXP. Unlike Rep, RepV196A was ClpXP sensitive without induced C-terminal flexibility. And unlike adaptor proteins that tether and deliver substrates to ClpX for trans-targeting, Vir promoted rapid degradation of Rep by ClpX deleted for the tethering site that binds adaptor proteins. Therefore, Rep's ClpX recognition motif has regulable properties, allowing an alternative trans-targeting mechanism in which an inactive degradation tag is turned on by induced conformational changes.

References

Oct 29, 1980·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R Schwyzer
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
Apr 19, 1996·The Journal of Biological Chemistry·P RousseauR Alazard
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·R W KriwackiP E Wright
Sep 23, 1997·Journal of Molecular Biology·D J WeltyH Nakai
Apr 18, 1998·European Journal of Biochemistry·R AlazardM Chandler
May 13, 1999·The Journal of Biological Chemistry·M Gonciarz-SwiatekM Zylicz
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S K SinghM R Maurizi
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·B G ReidE U Weber-Ban
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·C RanquetA Toussaint
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·J M FlynnT A Baker
Sep 14, 2001·Journal of Molecular Biology·S S RaiH Nakai
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Joel R HoskinsSue Wickner
Nov 9, 2002·The Journal of Biological Chemistry·Kimberly R Marshall-Batty, Hiroshi Nakai
Dec 18, 2002·Journal of Bacteriology·Dieter WeichartRegine Hengge-Aronis
Feb 13, 2003·Current Opinion in Structural Biology·Andreas Matouschek
May 6, 2003·Genes & Development·Saskia B NeherTania A Baker
Aug 26, 2003·The Journal of Biological Chemistry·Urszula A WojtyraWalid A Houry
Oct 16, 2003·The Journal of Biological Chemistry·Dawn A Defenbaugh, Hiroshi Nakai
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Saskia B NeherTania A Baker

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.