Transactivation by Runt related factor-2 of matrix metalloproteinase-13 in astrocytes

Neuroscience Letters
Takeshi Takarada, Yukio Yoneda

Abstract

We have previously shown functional expression by osteoblasts of signaling machineries required for neurotransmission in the brain. In this study, we have evaluated possible functional expression of different osseous genes in the brain. In embryonic and adult mouse brains, mRNA expression was invariably seen for the master regulator of osteoblastic differentiation Runt related factor-2 (Runx2), in addition to the partner protein core binding factor-beta and their targets such as osteopontin (OPN) and matrix metalloproteinase-13 (MMP13), but not for collagen-I or osteocalcin. In pluripotent P19 progenitor cells, Runx2 mRNA expression was drastically increased along with mRNA expression of an astrocytic marker, but not with neuronal marker mRNA expression. Both mRNA and corresponding protein were detected for Runx2 in cultured rat neocortical astrocytes and astrocytic C6 glioma cells. In C6 glioma cells, transient overexpression of Runx2 significantly increased mRNA expression of MMP13, but not of OPN. Moreover, transient overexpression of Runx2 significantly increased luciferase activity in C6 glioma cells transfected with the reporter plasmid linked to a wild-type Runx2 binding element in the MMP13 promoter, but not in cells wi...Continue Reading

References

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Jul 19, 2008·Journal of Cellular Physiology·Jae-Hwan JeongJe-Yong Choi

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Citations

Apr 29, 2014·Neurochemistry International·Ryota NakazatoYukio Yoneda
Aug 24, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jane B LianGary S Stein
Jan 3, 2015·DNA and Cell Biology·Daniel WysokinskiJanusz Blasiak
Jul 28, 2016·Biochemical and Biophysical Research Communications·Ji-Soo ParkJung-Eun Kim
Aug 5, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yukio Yoneda
Jul 22, 2018·In Vitro Cellular & Developmental Biology. Animal·Isha Verma, Polani B Seshagiri

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