PMID: 9557678Apr 29, 1998Paper

Transactivation-competent bovine papillomavirus E2 protein is specifically required for efficient repression of human papillomavirus oncogene expression and for acute growth inhibition of cervical carcinoma cell lines

Journal of Virology
E C GoodwinD DiMaio

Abstract

The papillomavirus E2 proteins can function as sequence-specific transactivators or transrepressors of transcription and as cofactors in viral DNA replication. We previously demonstrated that acute expression of the bovine papillomavirus type 1 (BPV1) E2 protein in HeLa and HT-3 cervical carcinoma cell lines greatly reduced cellular proliferation by imposing a specific G1/S phase growth arrest. In this report, we analyzed the effects of a panel of point mutations in the BPV1 E2 protein to identify the functional requirements for acute growth inhibition. Disruption of E2-specific transactivation by mutations within either the transactivation domain or the DNA binding domain severely impaired E2-mediated growth inhibition in HeLa and HT-3 cells, even though these mutants retain various other E2 activities. This result indicates that functional transactivation activity is required for acute E2-mediated growth inhibition. HeLa cells, which contain a wild-type p53 gene, and HT-3 cells, which contain a transactivation-defective p53 gene, exhibited similar responses to the E2 mutants, suggesting that identical functions of the E2 protein were required for growth arrest regardless of p53 status. Replacement of the E2 transactivation do...Continue Reading

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Citations

Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C GoodwinE S Hwang
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C Goodwin, D DiMaio
May 22, 2002·Journal of Virology·Frederick A Dick, Nicholas J Dyson
Jun 12, 2003·The Journal of Biological Chemistry·Mounia ChamiRosario Rizzuto
Apr 30, 2003·Journal of Virology·Allison H S Hall, Kenneth A Alexander
May 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Susanne I WellsPeter M Howley
Dec 13, 2005·Cancer Gene Therapy·E J Shillitoe
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jennifer A SmithPeter M Howley
May 23, 2002·The Journal of Biological Chemistry·Daeyoup LeeJoonho Choe
Apr 3, 2001·Journal of Cutaneous Medicine and Surgery·J SeversonS K Tyring
Sep 20, 2002·The Journal of Biological Chemistry·Samuel Y HouCheng-Ming Chiang
Apr 20, 2012·Journal of Virology·Thomas G MagaldiDaniel DiMaio
Oct 12, 2011·Protoplasma·R NascimentoR M E Parkhouse
Feb 29, 2008·Archives of Virology·Agnieszka K Olejnik-SchmidtAnna Goździcka-Józefiak
Feb 26, 2010·Journal of Virology·Maria L C PowellPeter M Howley
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Aug 7, 2002·Journal of Virology·Yijan E ChangLaimonis A Laimins
Jul 13, 2007·Journal of Virology·Michal-Ruth SchweigerPeter M Howley

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