Transactivation of the epidermal growth factor receptor mediates muscarinic stimulation of focal adhesion kinase in intestinal epithelial cells

Journal of Cellular Physiology
Sean O CalandrellaStephen J Keely

Abstract

We have previously shown that the Gq protein coupled receptor (GqPCR) agonist, carbachol (CCh), transactivates and recruits epidermal growth factor receptor (EGFr)-dependent signaling mechanisms in intestinal epithelial cells. Increasing evidence suggests that GqPCR agonists can also recruit focal adhesion-dependent signaling pathways in some cell types. Therefore, the aim of the present study was to investigate if CCh stimulates activation of the focal adhesion-associated protein, focal adhesion kinase (FAK), in intestinal epithelia and, if so, to examine the signaling mechanisms involved. Experiments were carried out on monolayers of T84 cells grown on permeable supports. CCh rapidly induced tyrosine phosphorylation of FAK in T84 cells. This effect was accompanied by phosphorylation of another focal adhesion-associated protein, paxillin, and association of FAK with paxillin. CCh-stimulated FAK phosphorylation was inhibited by a chelator of intracellular Ca2+, BAPTA/AM (20 microM), and was mimicked by thapsigargin (2 microM), which mobilizes intracellular Ca2+ in a receptor-independent fashion. CCh also induced association of FAK with the EGFr and FAK phosphorylation was attenuated by an EGFr inhibitor, tyrphostin AG1478, and ...Continue Reading

References

Mar 1, 1984·The American Journal of Physiology·S GrinsteinE W Gelfand
Mar 24, 1995·Science·A Levitzki, A Gazit
May 2, 1997·The Journal of Biological Chemistry·D C GamettI Friedberg
Aug 8, 1998·Trends in Cell Biology·D D Schlaepfer, T Hunter
Jan 16, 1999·Frontiers in Bioscience : a Journal and Virtual Library·L A Cary, J L Guan
Jun 4, 1999·Progress in Biophysics and Molecular Biology·D D SchlaepferD J Sieg
Dec 2, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J L Rodríguez-Fernández
Dec 14, 1999·The Journal of Biological Chemistry·J W ThomasM D Schaller
May 12, 2000·Nature Cell Biology·D J SiegD D Schlaepfer
Jun 10, 2000·The Journal of Biological Chemistry·I CastagliuoloC Pothoulakis
Jun 23, 2000·The Journal of Surgical Research·O J HinesD McFadden
Sep 8, 2000·The Journal of Biological Chemistry·L BarberisF G Giancotti
Sep 15, 2000·The Journal of Biological Chemistry·M A Sanders, M D Basson
Nov 18, 2000·Experimental Cell Research·S K Sastry, K Burridge
Jun 30, 2001·Trends in Pharmacological Sciences·M J Marinissen, J S Gutkind
Jul 31, 2001·Biochimica Et Biophysica Acta·M D Schaller
Dec 17, 2002·Journal of Applied Physiology·Yasushi ShikataJoe G N Garcia
Mar 18, 2003·Journal of Cell Science·J Thomas Parsons
Jul 8, 2003·The Journal of Pathology·Erik H J Danen, Arnoud Sonnenberg
Aug 7, 2003·Nephron. Experimental Nephrology·Ambra Pozzi, Roy Zent
Oct 16, 2003·The Journal of Biological Chemistry·Lindsay DavidsonStuart Maudsley

❮ Previous
Next ❯

Citations

Aug 2, 2006·Autonomic & Autacoid Pharmacology·R M Eglen
Apr 23, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·M Pelissier-RotaM R Jacquier-Sarlin
Jun 19, 2008·Pharmacology & Therapeutics·Neil M Nathanson
Apr 4, 2008·American Journal of Physiology. Cell Physiology·Alan L LinChih-Ko Yeh
Feb 28, 2007·Journal of Pediatric Gastroenterology and Nutrition·Maria A CarlosMrinalini C Rao
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yi-Ping JinElaine F Reed
Jun 20, 2020·American Journal of Physiology. Cell Physiology·Kelli JohnsonMark Donowitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.