Transaminase activity and other enzymatic reactions involving pyruvate and glutamate in Chlamydia (psittacosis-trachoma group).

Journal of Bacteriology
E Weiss

Abstract

The agents of meningopneumonitis (MN) and of trachoma (TR) purified from chick embryo allantoic fluids and yolk sacs, respectively, were shown to produce CO(2) from the C(1) positions of pyruvate and glutamate, but not from the other carbon atoms. The reaction with pyruvate did not require did not require the addition of cofactors, but was stimulated to a small extent by alpha-lipoic acid and, in the case of TR, also by diphosphothiamine, and nicotinamide adenine dinucleotide (NAD). The reaction of MN with glutamate was greatly stimulated by the addition of NAD and pyruvate, and resulted in the accumulation of alanine. The reaction of TR with glutamate was also greatly enhanced by added NAD, but was not affected by added pyruvate. When eight intermediates of the citric acid cycle were added to MN cells incubated with glutamate-C(14), plus NAD and pyruvate, they reduced to varying degrees the evolution of C(14)O(2). It was shown by chromatography that the C(14) label extended to alpha-ketoglutarate and succinate, but not to fumarate and malate. A net gain in adenosine triphosphate could not be demonstrated in MN cells incubated with combined glutamate, pyruvate, oxaloacetate, and various cofactors. These results furnish addition...Continue Reading

References

Jun 1, 1966·The Journal of Infectious Diseases·H S Lin, J W Moulder
Aug 1, 1962·Analytical Biochemistry·F SNYDER, N STEPHENS
Mar 1, 1962·The Journal of Infectious Diseases·E WEISSE M NEPTUNE
Mar 1, 1963·Journal of Bacteriology·J W MOULDERI C TRIBBY
Mar 1, 1965·Journal of Bacteriology·J W MOULDERR R BRUBAKER
Apr 1, 1965·The Journal of Infectious Diseases·F B GORDON, A L QUAN
Jun 1, 1949·The Journal of Experimental Medicine·M R BOVARNICK, J C SNYDER

❮ Previous
Next ❯

Citations

Sep 1, 1992·Avian Pathology : Journal of the W.V.P.A·T Suwa, C Itakura
Jun 9, 2017·MSystems·Lena KönigMatthias Horn
Oct 13, 2000·Molecular Microbiology·E R Iliffe-Lee, G McClarty
Oct 1, 1968·Journal of Bacteriology·D T Kingsbury, E Weiss
Jul 1, 1979·Infection and Immunity·M M Ceballos, T P Hatch
May 22, 2019·Microbiology Spectrum·Marion RotherThomas Rudel
Jun 1, 1969·Journal of Bacteriology·D T Kingsbury
Sep 15, 1974·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J I BruceJ W Crum
Nov 1, 1969·Journal of Bacteriology·R W GauglerN N Wilson
Mar 1, 1991·Microbiological Reviews·J W Moulder
Jan 1, 1970·Journal of Bacteriology·L P Mallavia, E Weiss
Jun 1, 1971·Experimental Parasitology·I W ShermanI P Ting

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.