Transbilayer exchange of phosphatidylethanolamine for phosphatidylcholine and N-acetimidoylphosphatidylethanolamine in single-walled bilayer vesicles.

Biochemistry
M A RosemanT E Thompson

Abstract

A preparation of small single-walled liposome vesicles containing a 9:1 mole ratio of phosphatidylcholine to phosphatidylethanolamine was subjected to reaction with the membrane-impermeable reagent, isethionyl acetimidate hydrochloride. This reagent converted 90% of the external phosphatidylethanolamine groups to the amidine derivative, leaving the mole ratio of unreacted phosphatidylethanolamine to phosphatidylcholine on the outside surface of the vesicle much lower than that on the inside surface. Equilibration of phosphatidylethanolamine across the bilayer was then measured as a function of time by monitoring the appearance of phosphatidylethanolamine on the outside surface utilizing the reaction of the amino groups with 2, 4, 6-trinitrobenzenesulfonic acid. The results show that no new phosphatidylethanolamine appeared on the external surface of the vesicles over a period of 12 days at 22 degrees. A conservative estimate of the precision of the measurements is +/- 10%. On this basis, the estimated half-time for the equilibration of phosphatidylethanolamine across the bilayer of these vesicles must be at least 80 days at 22 degrees.

Citations

Jan 1, 1977·Journal of Supramolecular Structure·S J Singer
Sep 1, 1982·Biulleten' eksperimental'noĭ biologii i meditsiny·O A RozenbergK P Khanson
May 13, 1981·Biochimica Et Biophysica Acta·P B Kingsley, G W Feigenson
Aug 4, 1994·Biochimica Et Biophysica Acta·P J Trotter, D R Voelker
Feb 1, 1980·Chemistry and Physics of Lipids·B Liljenfors, H Löfgren
Jan 1, 1981·Progress in Biophysics and Molecular Biology·P J Quinn
Jul 31, 1978·Biochimica Et Biophysica Acta·R F Zwaal
Jan 3, 1978·Biochimica Et Biophysica Acta·R L DormerA K Campbell
Feb 29, 2000·Biochimica Et Biophysica Acta·R P Huijbregts de Kruijff B
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E Rothman, E P Kennedy
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R SundlerP R Vagelos
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K E Langley, E P Kennedy
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G KaplanZ A Cohn
Mar 8, 1979·Biochimica Et Biophysica Acta·J M Backer, E A Dawidowicz
Dec 14, 1976·Biochimica Et Biophysica Acta·D A TyrrellB E Ryman
Feb 13, 2010·Journal of Liposome Research·Chantal J DicaireG Dennis Sprott
Jan 1, 1979·CRC Critical Reviews in Biochemistry·E J PatzerE J Dubovi
Apr 1, 1979·European Journal of Biochemistry·E J DemantL L van Deenen
Sep 19, 1977·Biochimica Et Biophysica Acta·G Duckwitz-peterleinP Overath
Jan 25, 1984·Biochimica Et Biophysica Acta·A Kumar, C M Gupta
Jul 28, 1982·Biochimica Et Biophysica Acta·S J DeardenJ Philp
Mar 17, 1977·Biochimica Et Biophysica Acta·A M van den BesselaarL L van Deenen
Feb 20, 1979·Biochimica Et Biophysica Acta·D G BishopL L van Deenen
Mar 1, 1977·Biochimica Et Biophysica Acta·P L YeagleR B Martin
Jan 1, 1981·International Journal of Nuclear Medicine and Biology·J A Rogers, A A Noujaim
Jul 1, 1982·Journal of Neurochemistry·M Butler, P Morell
Mar 5, 1977·Journal of Molecular Biology·J E Rothman, E P Kennedy
Dec 14, 2016·Accounts of Chemical Research·John S Allhusen, John C Conboy
Feb 26, 1985·Biochemistry·S F Greenhut, M A Roseman

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