Transcript and proteomic analysis of developing white lupin (Lupinus albus L.) roots.

BMC Plant Biology
Li TianRichard A Dixon

Abstract

White lupin (Lupinus albus L.) roots efficiently take up and accumulate (heavy) metals, adapt to phosphate deficiency by forming cluster roots, and secrete antimicrobial prenylated isoflavones during development. Genomic and proteomic approaches were applied to identify candidate genes and proteins involved in antimicrobial defense and (heavy) metal uptake and translocation. A cDNA library was constructed from roots of white lupin seedlings. Eight thousand clones were randomly sequenced and assembled into 2,455 unigenes, which were annotated based on homologous matches in the NCBInr protein database. A reference map of developing white lupin root proteins was established through 2-D gel electrophoresis and peptide mass fingerprinting. High quality peptide mass spectra were obtained for 170 proteins. Microsomal membrane proteins were separated by 1-D gel electrophoresis and identified by LC-MS/MS. A total of 74 proteins were putatively identified by the peptide mass fingerprinting and the LC-MS/MS methods. Genomic and proteomic analyses identified candidate genes and proteins encoding metal binding and/or transport proteins, transcription factors, ABC transporters and phenylpropanoid biosynthetic enzymes. The combined EST and pr...Continue Reading

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Citations

Mar 29, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Muhammad Javed IqbalHarbans L Bhardwaj
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Datasets Mentioned

BETA
AAR08412

Methods Mentioned

BETA
cDNA library construction
electrophoresis
PCR

Software Mentioned

Primer3
BLAST
PhpSSRMiner
tBLASTx
TGICL
SSRIT
Mascot
BLASTX
IsPCR

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