Transcript elongation factor TFIIS is involved in arabidopsis seed dormancy

Journal of Molecular Biology
Marion GrasserKlaus D Grasser

Abstract

Transcript elongation factor TFIIS promotes efficient transcription by RNA polymerase II, since it assists in bypassing blocks during mRNA synthesis. While yeast cells lacking TFIIS are viable, inactivation of mouse TFIIS causes embryonic lethality. Here, we have identified a protein encoded in the Arabidopsis genome that displays a marked sequence similarity to TFIIS of other organisms, primarily within domains II and III in the C-terminal part of the protein. TFIIS is widely expressed in Arabidopsis, and a green fluorescent protein-TFIIS fusion protein localises specifically to the cell nucleus. When expressed in yeast cells lacking the endogenous TFIIS, Arabidopsis TFIIS partially complements the sensitivity of mutant cells to the nucleotide analog 6-azauridine, which is a typical characteristic of transcript elongation factors. We have characterised Arabidopsis lines harbouring T-DNA insertions in the coding sequence of TFIIS. Plants homozygous for T-DNA insertions are viable, and genomewide transcript profiling revealed that compared to control plants, a relatively small number of genes are differentially expressed in mutant plants. TFIIS(-/-) plants display essentially normal development, but they flower slightly earlier ...Continue Reading

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Citations

May 1, 2012·Nucleic Acids Research·Fernando Gómez-HerrerosSebastián Chávez
Aug 19, 2014·Trends in Plant Science·Mieke Van Lijsebettens, Klaus D Grasser
Dec 8, 2015·Plant & Cell Physiology·Naoto SanoMitsunori Seo
Aug 26, 2014·The Plant Journal : for Cell and Molecular Biology·Martijn van ZantenWim J J Soppe
Dec 2, 2009·The Plant Journal : for Cell and Molecular Biology·Ihab B LolasKlaus D Grasser
Oct 7, 2015·Frontiers in Plant Science·Irene MurgiaPiero Morandini
Apr 15, 2014·Molecular Cell·Sebastian MarquardtCaroline Dean
Jun 7, 2014·Frontiers in Plant Science·Hiroyuki Nonogaki
Jan 9, 2015·The EMBO Journal·Jakub DolataSzymon Świeżewski
Jun 5, 2020·Plants·Gerardo Carrera-CastañoLuis Oñate-Sánchez
Aug 5, 2017·Plant, Cell & Environment·Laura Narro-DiegoManuel Piñeiro
Jun 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·Xiaofeng FangCaroline Dean
May 21, 2019·Frontiers in Plant Science·Wesam M Al KhateebDana F Schroeder
Nov 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Halina FedakSzymon Swiezewski
May 1, 2021·International Journal of Molecular Sciences·Elodie LayatAline V Probst

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