Transcript level is a key factor affecting RNAi efficiency.

Pesticide Biochemistry and Physiology
Jiasheng ChenZ Han

Abstract

Efficiency is the basis for the application of RNA interference (RNAi) technology. Actually, RNAi efficiency varies greatly among insect species, tissues and genes. Previous efforts have revealed the mechanisms for variation among insect species and tissues. Here, we investigated the reason for variable efficiency among the target genes in the same insect. First, we tested the genes sampled randomly from Tribolium castaneum, Locusta migratoria and Drosophila S2 cells for both their expression levels and sensitivity to RNAi. The results indicated that the genes with higher expression levels were more sensitive to RNAi. Statistical analysis showed that the correlation coefficients between transcript levels and knockdown efficiencies were 0.8036 (n = 90), 0.7255 (n = 18) and 0.9505 (n = 13), respectively in T. castaneum, L. migratoria and Drosophila S2 cells. Subsequently, ten genes with varied expression level in different tissues (midgut and carcass without midgut) of T. castaneum were tested. The results indicated that the higher knockdown efficiency was always obtained in the tissue where the target gene expressed higher. In addition, three genes were tested in different developmental stages, larvae and pupae of T. castaneum. ...Continue Reading

References

Jul 12, 2002·Nature·Gregory J Hannon
Sep 17, 2004·Nature·David Baulcombe
Jul 25, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K S VinokurovM A Belozersky
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·C P HunterM C Fitzgerald
Nov 6, 2007·Nature Biotechnology·James A BaumJames Roberts
Dec 8, 2009·Annual Review of Entomology·Xavier Bellés
Jul 6, 2010·Insect Biochemistry and Molecular Biology·Shuhua LiuZewen Liu
Feb 8, 2011·Insect Biochemistry and Molecular Biology·Bart BoerjanRoger Huybrechts
Aug 3, 2011·Journal of Neurochemistry·Ermelinda LomazzoKenneth J Kellar
Oct 2, 2012·Insect Biochemistry and Molecular Biology·Niels WynantJozef Vanden Broeck
Dec 12, 2012·Cellular and Molecular Life Sciences : CMLS·Guowen DuanKarl H J Gordon
Feb 13, 2014·ELife·Cristina Cruz, Jonathan Houseley
May 20, 2015·Pesticide Biochemistry and Physiology·Young Ho KimKun Yan Zhu
Jul 28, 2016·Insect Biochemistry and Molecular Biology·Kangxu WangZhaojun Han
Jun 4, 2017·Insect Biochemistry and Molecular Biology·Huifang SongJianzhen Zhang
Jul 29, 2017·Cell·Aviad TsherniakWilliam C Hahn
Aug 22, 2017·Trends in Biotechnology·Jiang ZhangRalph Bock
Feb 21, 2018·Nature Communications·Maja M JanasVasant Jadhav
Mar 20, 2018·Cell·Cristina HoehenerMariusz Nowacki
Jun 13, 2018·Frontiers in Physiology·Yingchuan PengZhaojun Han
Jun 23, 2018·Pest Management Science·Anastasia Mw CooperKun Yan Zhu
Jul 7, 2018·Annual Review of Phytopathology·Cristina RosaBryce W Falk
Aug 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·June-Sun YoonSubba Reddy Palli
Oct 10, 2018·Nature Communications·Huan-Huan QiaoJian-Quan Ni
Jan 9, 2019·Frontiers in Physiology·Ruobing GuanXuexia Miao
Oct 15, 2019·Annual Review of Entomology·Kun Yan Zhu, Subba Reddy Palli
Nov 5, 2019·Biotechnology Advances·Shaoshuai LiuKarl-Heinz Kogel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.