Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers

Genomics
A G ElkahlounP S Meltzer

Abstract

We used a combination of sequence analysis and exon trapping in an effort to determine the complete transcript map for a cosmid (6E5) derived from 12q13.3, a region of DNA sequence amplification in human cancers. This cosmid, previously known to contain three genes (CDK4, SAS, and OS9), was sequenced, and that information was used for computer-assisted analysis. In addition, 6E5 was subjected to both internal and 3'-terminal exon-trapping protocols, and the results of these studies were used to guide cDNA cloning experiments. These studies demonstrate that this cosmid is derived from a remarkably gene-dense region and add two new transcripts (KIAA0167 and 6E5.2) to the list of sequences that are expressed in tumors bearing amplification of this region.

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Citations

Jun 19, 2013·Acta Pharmacologica Sinica·Qi Qi, Keqiang Ye
Mar 26, 2009·The Journal of Biological Chemistry·Yunjuan ZhuZhongzhen Nie
Feb 27, 2013·Molecular and Cellular Biology·Margaret Chui Ling TseChi Bun Chan
Jun 14, 2005·International Journal of Biological Sciences·Jee-Yin Ahn, Keqiang Ye
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·H HermekingK W Kinzler
May 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jee-Yin AhnKeqiang Ye
Sep 1, 2015·Journal of Cancer Research and Clinical Oncology·Li TanHuo Tan
Aug 10, 2005·Journal of Cellular Biochemistry·Keqiang Ye
Mar 21, 2007·Journal of Cellular and Molecular Medicine·Chi Bun Chan, Keqiang Ye
Aug 10, 1999·Genes, Chromosomes & Cancer·W V YotovE Levy
Jun 20, 2008·Clinical Orthopaedics and Related Research·Ni TangTong-Chuan He
Feb 26, 2020·International Journal of Molecular Sciences·Amaia Navarro-CorcueraMaría J Iraburu

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