Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus.

PloS One
Hao ChenXiaoxin Chen

Abstract

Morphological changes during human and mouse esophageal development have been well characterized. However, changes at the molecular level in the course of esophageal morphogenesis remain unclear. This study aims to globally profile critical genes and signaling pathways during the development of mouse esophagus. By using microarray analysis this study also aims to determine how the Nrf2/Keap1 pathway regulates the morphogenesis of the esophageal epithelium. Gene expression microarrays were used to survey gene expression in the esophagus at three critical phases: specification, metaplasia and maturation. The esophagi were isolated from wild-type, Nrf2(-/-), Keap1(-/-), or Nrf2(-/-)Keap1(-/-) embryos or young adult mice. Array data were statistically analyzed for differentially expressed genes and pathways. Histochemical and immunohistochemical staining were used to verify potential involvement of the Wnt pathway, Pparβ/δ and the PI3K/Akt pathway in the development of esophageal epithelium. Dynamic gene expression patterns accompanied the morphological changes of the developing esophagus at critical phases. Particularly, the Nrf2/Keap1 pathway had a baseline activity in the metaplasia phase and was further activated in the maturat...Continue Reading

References

Jun 1, 1991·The Anatomical Record·C RaymondG Millane
Jun 15, 1995·Microscopy Research and Technique·D Ménard
Sep 10, 1998·Nature Genetics·Y LitingtungC Chiang
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Jun 1, 1999·Methods in Enzymology·M B Eisen, P O Brown
May 12, 2001·The Journal of Investigative Dermatology·I M FreedbergM Blumenberg
May 12, 2001·The Journal of Investigative Dermatology·M WestergaardK Kristiansen
Feb 7, 2002·Journal of Dental Research·R Todd, D T W Wong
Apr 5, 2002·Genome Research·W James Kent
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Silvia MarettoStefano Piccolo
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elva DíazJohn Ngai
Oct 1, 2003·Nature Genetics·Nobunao WakabayashiMasayuki Yamamoto
Apr 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hozumi MotohashiMasayuki Yamamoto
Dec 18, 2004·Development·Aileen McDermottAnne-Gaëlle Borycki
Apr 27, 2005·Journal of Bioinformatics and Computational Biology·Jeff W ChouPierre R Bushel
Jul 16, 2005·Cell Death and Differentiation·D J KimJ M Peters
Sep 14, 2006·Annual Review of Pharmacology and Toxicology·Thomas W KenslerShyam Biswal
Feb 15, 2007·Expert Opinion on Therapeutic Targets·Geneviève ClémentJean Benhattar
Aug 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michelle L KernsPierre A Coulombe
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xiu Jun WangC Roland Wolf
Dec 20, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Richard I SherwoodDouglas A Melton
May 27, 2009·Journal of Gastroenterology·Jacob WangXiaoxin Chen
Aug 14, 2009·The Journal of Investigative Dermatology·Yan J JiangKenneth R Feingold
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kelley S Harris-JohnsonXin Sun
Feb 16, 2010·Developmental Cell·Edward E Morrisey, Brigid L M Hogan
Mar 11, 2010·Science Signaling·Gerasimos P Sykiotis, Dirk Bohmann
Mar 20, 2010·PloS One·Malgorzata RomanowskaJohn Foerster
Jul 16, 2010·Science Signaling·Nobunao WakabayashiThomas W Kensler

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Citations

Jan 16, 2016·Oxidative Medicine and Cellular Longevity·Hilal S KhalilYusuf Y Deeni
Oct 15, 2013·Annals of the New York Academy of Sciences·Andrew M BellizziXiaoxin Chen
May 3, 2014·Tissue Barriers·Hao ChenXiaoxin Luke Chen
Mar 17, 2015·The Journal of Clinical Investigation·Ming JiangJianwen Que
Dec 3, 2015·PloS One·Hao ChenXiaoxin Chen
Nov 10, 2018·The Journal of Biological Chemistry·Junsheng FuXiaoxin Chen
Apr 17, 2020·Birth Defects Research·Chantal A Ten KateErwin Brosens
May 13, 2018·Annals of the New York Academy of Sciences·Shaohua MaXiaoxin Luke Chen
Jan 11, 2017·Molecular Cancer Therapeutics·Nathaniel Edward Bennett SaiduJérôme Alexandre
Jul 26, 2013·Development·Ming JiangJianwen Que

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Datasets Mentioned

BETA
GSE34278

Methods Mentioned

BETA
nuclear translocation
PCR
Assay
PCA
transgenic

Software Mentioned

SAM
constrast
SDS
GeneSpring
RQ Manager
GSEA
R package samr
ExP
GSA
Excel

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