Transcript profiling of candidate genes in testis of pigs exhibiting large differences in androstenone levels.

BMC Genetics
E GrindflekS Lien

Abstract

Boar taint is an unpleasant odor and flavor of the meat and occurs in a high proportion of uncastrated male pigs. Androstenone, a steroid produced in testis and acting as a sex pheromone regulating reproductive function in female pigs, is one of the main compounds responsible for boar taint. The primary goal of the present investigation was to determine the differential gene expression of selected candidate genes related to levels of androstenone in pigs. Altogether 2560 boars from the Norwegian Landrace and Duroc populations were included in this study. Testicle samples from the 192 boars with most extreme high or low levels of androstenone in fat were used for RNA extraction, and 15 candidate genes were selected and analyzed by real-competitive PCR analysis. The genes Cytochrome P450 c17 (CYP17A1), Steroidogenic acute regulatory protein (STAR), Aldo-keto reductase family 1 member C4 (AKR1C4), Short-chain dehydrogenase/reductase family member 4 (DHRS4), Ferritin light polypeptide (FTL), Sulfotransferase family 2A, dehydroepiandrosterone-preferring member 1 (SULT2A1), Cytochrome P450 subfamily XIA polypeptide 1 (CYP11A1), Cytochrome b5 (CYB5A), and 17-beta-Hydroxysteroid dehydrogenase IV (HSD17B4) were all found to be significa...Continue Reading

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Citations

Oct 26, 2010·BMC Genomics·Yuliaxis Ramayo-CaldasJosep M Folch
Nov 30, 2010·Journal of Proteomics·Emøke BendixenIngrid Miller
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Oct 30, 2018·Molecular Reproduction and Development·Ying LiuKui Li
Jan 7, 2021·Journal of Applied Genetics·Darlene Ana Souza DuarteNicolas Gengler

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Methods Mentioned

BETA
Assay
PCR
differential
transcription profiling
genotyping

Software Mentioned

MassARRAY Assay Design
MassARRAY QGE Assay Design
MassARRAY
MassARRAY Typer
Genotype Analyzer
SAS
rcPCR
R
TITAN
iPLEX

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