Transcript-specific translational regulation in the unfolded protein response of Saccharomyces cerevisiae

FEBS Letters
Tom PayneDavid B Archer

Abstract

Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes stress and induces the unfolded protein response (UPR). Genome-wide analysis of translational regulation in response to the UPR-inducing agent dithiothreitol in Saccharomyces cerevisiae is reported. Microarray analysis, confirmed using qRT-PCR, identified transcript-specific translational regulation. Transcripts with functions in ribosomal biogenesis and assembly were translationally repressed. In contrast, mRNAs from known UPR genes, encoding the UPR transcription factor Hac1p, the ER-oxidoreductase Ero1p and the ER-associated protein degradation (ERAD) protein Der1p, were enriched in polysomal fractions, indicating translational up-regulation. Splicing of HAC1 mRNA is shown to be required for efficient ribosomal loading.

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Citations

Oct 13, 2009·Journal of Industrial Microbiology & Biotechnology·Bing-Zhi LiYing-Jin Yuan
Mar 30, 2010·BMC Genomics·Martin DragositsDiethard Mattanovich
Jun 4, 2015·Current Opinion in Structural Biology·Roslyn M Bill, Tobias von der Haar
Jul 18, 2014·Current Opinion in Biotechnology·Carissa L Young, Anne S Robinson
Jan 21, 2015·Biochemical and Biophysical Research Communications·Esther Weindling, Shoshana Bar-Nun
Oct 22, 2016·PLoS Genetics·Lasse van WijlickJoachim F Ernst
Jun 20, 2019·International Journal of Molecular Sciences·Xuhua Xia
Jun 21, 2019·Metabolic Engineering·Pamela B Besada-Lombana, Nancy A Da Silva

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