Transcription activation at promoters carrying tandem DNA sites for the Escherichia coli cyclic AMP receptor protein: organisation of the RNA polymerase alpha subunits

Journal of Molecular Biology
T A BelyaevaS J Busby

Abstract

We have constructed a family of promoters carrying tandem DNA sites for the Escherichia coli cyclic AMP receptor protein (CRP), with one of the sites centred between base-pairs 41 and 42 upstream from the transcription start site, and the second site located further upstream. In vivo activity measurements show that the activity of these promoters is completely dependent on CRP and that, depending on the precise location, CRP bound at the upstream site increases transcription activation. Hydroxyl radical footprinting was exploited to investigate the binding of CRP and RNA polymerase holoenzyme (RNAP) to these promoters. The study shows that the C-terminal domains of the RNAP alpha subunits bind adjacent to the upstream CRP and that their precise positioning depends on the location of upstream-bound CRP. The C-terminal domains of the RNAP alpha subunits interact with both the upstream and downstream-bound CRP via activating region 1 of CRP.

References

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Citations

Mar 31, 2005·Current Opinion in Genetics & Development·Lacramioara BintuRob Phillips
Apr 23, 2004·Current Opinion in Structural Biology·Catherine L LawsonRichard H Ebright
Mar 24, 2004·Nature Reviews. Microbiology·Douglas F Browning, Stephen J Busby
Feb 26, 2000·Molecular Microbiology·R WassemM Buck
Feb 20, 2004·Nucleic Acids Research·Wilfried J J Meijer, Margarita Salas
Nov 3, 2004·Nucleic Acids Research·Dongling ZhengStephen D Minchin
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·R L GourseW Ross
Jul 28, 2009·Journal of Bacteriology·Seyyed I HusnainMark S Thomas
Nov 4, 2008·Journal of Bacteriology·Julio Collado-VidesAlberto Santos-Zavaleta
Oct 4, 2003·Journal of Bacteriology·Anne M L BarnardStephen J W Busby
Sep 13, 2012·MBio·David J Lee, Stephen J W Busby
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·R C Langdon, A Hochschild
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·S E AiyarW Ross
Jul 20, 1999·Molecular Microbiology·M VicenteV De Lorenzo
Jun 26, 2012·Annual Review of Microbiology·David J LeeStephen J W Busby
Nov 13, 2003·FEMS Microbiology Letters·Sarah L Messenger, Jeffrey Green
Jan 18, 2018·Applied Microbiology and Biotechnology·Peng ZhaoPingfang Tian

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