PMID: 9547262Jun 20, 1998Paper

Transcription activation by Escherichia coli FNR protein: similarities to, and differences from, the CRP paradigm

Nucleic Acids Research
B LiS Busby

Abstract

During transcription activation at FNR-dependent promoters where the DNA site for FNR overlaps the -35 element, a surface-exposed activating region in the upstream subunit of the FNR dimer interacts with the C-terminal domain of the RNA polymerase alpha subunit. Starting with a cloned fnr gene encoding a defective FNR derivative carrying substitutions in this activating region, we screened a library of random mutations to identify substitutions that restored FNR activity. Activity can be restored by substitutions at residues T118, E47 and K60. The locations of these residues identify three separate surface-exposed regions of FNR that can play a role in transcription activation. These three regions appear to be analogues of Activating Region 1, Activating Region 2 and Activating Region 3 of the cyclic AMP receptor protein, CRP: our results underscore the similarities between FNR and CRP.

References

Aug 15, 1992·FEMS Microbiology Letters·J LodgeN R Kamini
Aug 1, 1990·FEMS Microbiology Reviews·S Spiro, J R Guest
Jan 1, 1993·Annual Review of Biochemistry·A KolbS Adhya
May 1, 1993·Molecular Microbiology·R H Ebright
Mar 1, 1997·Molecular Microbiology·S Busby, R H Ebright

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Citations

Jan 16, 2010·The Journal of Biological Chemistry·Anders BoysenMartin Overgaard
Mar 20, 2012·Antioxidants & Redox Signaling·Serena RinaldoFrancesca Cutruzzolà
Nov 3, 2004·Nucleic Acids Research·Dongling ZhengStephen D Minchin
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·S L Dove, A Hochschild
Dec 14, 2001·Journal of Bacteriology·Dinene L Crater, Charles P Moran
Oct 11, 2002·Journal of Bacteriology·Timo BlakeJeffrey Green
Mar 20, 2008·Journal of Molecular Microbiology and Biotechnology·M Julia PettinariBeatriz S Méndez
Dec 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Christian L BarrettBernhard O Palsson
Jun 26, 2012·Annual Review of Microbiology·David J LeeStephen J W Busby
Oct 26, 2005·Journal of Molecular Biology·Erin L Mettert, Patricia J Kiley
Apr 23, 2003·FEBS Letters·Colin ScottJeffrey Green
Sep 24, 2004·The Journal of Biological Chemistry·Stelian M PopStephen W Ragsdale
Dec 2, 2000·Microbiology·M F AnjumJ R Guest
Jan 10, 2002·The Journal of Biological Chemistry·Hyung-Sik WonBong-Jin Lee
Oct 11, 2017·Antioxidants & Redox Signaling·Erin L Mettert, Patricia J Kiley
Jul 10, 1999·Journal of Bacteriology·K H WadeC P Moran
Nov 5, 1999·Journal of Molecular Biology·S Busby, R H Ebright

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