Transcription activation by remodelling of a nucleoprotein assembly: the role of NarL at the FNR-dependent Escherichia coli nir promoter

Molecular Microbiology
D F BrowningStephen J W Busby

Abstract

Expression from the Escherichia coli nir promoter is co-dependent on both the FNR protein (an anaerobically triggered transcription activator) and NarL or NarP proteins (transcription activators triggered by nitrite and nitrate). We found previously that FNR binds to a site centred at position - 41.5 at the nir promoter, but that FNR-dependent activation is repressed by IHF binding to a site centred at position -88 (IHF I) and Fis binding to sites centred at positions -142 (Fis I) and +23 (Fis II). Here, we have studied the binding of purified IHF, Fis and FNR to the nir promoter in vitro. Our results show that the nir promoter contains a second IHF site at position -115 (IHF II) and a third Fis site at position -97 (Fis III), and that IHF, Fis and FNR can bind together to form multiprotein complexes. Surprisingly, IHF binding at the IHF II site increases FNR-dependent activation by decreasing the repression mediated by IHF and Fis binding at the other sites. In previous work, we found that NarL or NarP activates the nir promoter by binding to a site centred at position -69.5 and counteracting the repressive effects of IHF and Fis. We now show that NarL can displace IHF bound at the IHF I site, but IHF is unable to displace bou...Continue Reading

References

Nov 1, 1992·Molecular Microbiology·S E Finkel, R C Johnson
Aug 15, 1992·FEMS Microbiology Letters·J LodgeN R Kamini
Jan 30, 1999·Biochimie·T Bykowski, A Sirko
Jun 29, 2000·The Journal of Biological Chemistry·S M ArfinG W Hatfield
Apr 3, 2001·Current Opinion in Microbiology·S M McLeod, R C Johnson
Sep 28, 2002·Nature Structural Biology·Ann E MarisRichard E Dickerson
Oct 24, 2003·Current Opinion in Microbiology·Agustino Martínez-Antonio, Julio Collado-Vides

❮ Previous
Next ❯

Citations

Feb 13, 2007·Journal of Bacteriology·Erin L Mettert, Patricia J Kiley
Sep 2, 2008·Journal of Bacteriology·Douglas F BrowningStephen J W Busby
Jan 24, 2013·PLoS Genetics·Kiran ChintakayalaDavid C Grainger
Jun 26, 2012·Annual Review of Microbiology·David J LeeStephen J W Busby
Jun 5, 2007·Biophysical Chemistry·Chiara ZurlaDavid D Dunlap
Jan 20, 2007·Molecular Microbiology·Dalit Weinstein-Fischer, Shoshy Altuvia
Jun 11, 2010·The Biochemical Journal·David L ChismonStephen J W Busby
Jan 15, 2013·Biochemical and Biophysical Research Communications·Ana Varela-RaposoClaudina Rodrigues-Pousada
Aug 9, 2016·Nature Reviews. Microbiology·Douglas F Browning, Stephen J W Busby
Feb 19, 2019·Microbiology·Stephen J W Busby
Feb 1, 2012·Prikladnaia biokhimiia i mikrobiologiia·A M AnuchinA S Kaprel'iants
Sep 5, 2007·Microbiology·Meranda D BradleyRobert Osuna
May 20, 2020·Frontiers in Microbiology·Emilio BuenoFelipe Cava
Aug 1, 2009·EcoSal Plus·Akira Ishihama
Sep 1, 2008·EcoSal Plus·Jeffrey A Cole, David J Richardson
May 25, 2021·Frontiers in Molecular Biosciences·Sylvain Durand, Maude Guillier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.