PMID: 8602357Feb 1, 1996Paper

Transcription activation by the bacteriophage Mu Mor protein: analysis of promoter mutations in Pm identifies a new region required for promoter function

Nucleic Acids Research
I Artsimovitch, M M Howe

Abstract

Middle transcription of bacteriophage Mu requires Escherichia coli RNA polymerase holoenzyme and a Mu-encoded protein, Mor. Consistent with these requirements, the middle promoter, Pm, has a recognizable -10 region but lacks a -35 region. Mutagenesis of this promoter (from -70 to +10) was performed using mutagenic oligonucleotide-directed PCR. The resulting fragments were cloned into a promoter-lacZfusion vector and analyzed for promoter activity by assaying beta-galactosidase production. Single point mutations with a Down phenotype were clustered in three regions: the -10 region, the Mor footprint region and the spacer between them. Gel retardation experiments with purified Mor protein and promoter mutants demonstrated that sequences important for Mor binding are located within the Mor footprint region and lead us to propose the existence of a dyad symmetry element involved in Mor binding. In agreement with this prediction, glutaraldehyde crosslinking of Mor in solution generated a species with the size of a dimer. These experiments also identified an unusual group of mutations located in the spacer region adjacent to the Mor footprint. These mutations alter promoter activity without affecting Mor binding. A circular permutati...Continue Reading

References

Jul 25, 1975·Journal of Molecular Biology·A I Bukhari
Jan 1, 1992·DNA Sequence : the Journal of DNA Sequencing and Mapping·C A BaxaM M Howe
Jan 1, 1991·Methods in Enzymology·J Carey
Jan 1, 1990·Journal of Bacteriology·S F Stoddard, M M Howe
Dec 1, 1990·Journal of Bacteriology·K Mathee, M M Howe
Jun 1, 1989·Journal of Bacteriology·S F Stoddard, M M Howe
Oct 20, 1988·Nature·M Ptashne
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jan 1, 1985·Nephron·C M Kjellstrand
Jan 1, 1985·Annual Review of Biochemistry·W R McClure
Mar 14, 1974·Molecular & General Genetics : MGG·A Toussaint, J P Lecocq
Feb 28, 1970·Journal of Molecular Biology·A D RiggsS Bourgeois
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa
Sep 1, 1993·Journal of Bacteriology·K Mathee, M M Howe

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Citations

Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·I ArtsimovitchM M Howe
Aug 24, 2011·The Journal of Biological Chemistry·Muthiah KumaraswamiMartha M Howe
Sep 6, 2011·Nucleosides, Nucleotides & Nucleic Acids·Olga Nikolaevna KorolevaValeriy Lvovich Drutsa
Jul 11, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Karthik K ShanmuganathamHee-Won Park
Jun 7, 2003·Molecular Microbiology·Anne-Marie HansenDing Jun Jin
Jan 20, 2004·The Journal of Biological Chemistry·Muthiah KumaraswamiHee-Won Park
Mar 1, 1996·Journal of Bacteriology·M Kahmeyer-Gabbe, M M Howe

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